Gene description for Rap2c
Gene name RAP2C, member of RAS oncogene family
Gene symbol Rap2c
Other names/aliases 2010200P20Rik
AI194294
AL022976
Species Mus musculus
 Database cross references - Rap2c
ExoCarta ExoCarta_72065
Vesiclepedia VP_72065
Entrez Gene 72065
UniProt Q8BU31  
 Rap2c identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rap2c
Molecular Function
    transcription coactivator activity GO:0003713 IEA
    transcription coactivator activity GO:0003713 ISO
    GTPase activity GO:0003924 IBA
    G protein activity GO:0003925 IEA
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IBA
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
Biological Process
    negative regulation of cell migration GO:0030336 IBA
    negative regulation of cell migration GO:0030336 IDA
    positive regulation of protein autophosphorylation GO:0031954 IDA
    Rap protein signal transduction GO:0032486 IBA
    Rap protein signal transduction GO:0032486 IDA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    regulation of protein tyrosine kinase activity GO:0061097 IDA
    establishment of endothelial intestinal barrier GO:0090557 IEA
    establishment of endothelial intestinal barrier GO:0090557 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    bicellular tight junction GO:0005923 IEA
    bicellular tight junction GO:0005923 ISO
    membrane GO:0016020 IDA
    cell-cell contact zone GO:0044291 IEA
    cell-cell contact zone GO:0044291 ISO
    recycling endosome GO:0055037 IDA
    recycling endosome membrane GO:0055038 IDA
 Experiment description of studies that identified Rap2c in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rap2c
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Usp7  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Rap2c is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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