Gene description for UBE2H
Gene name ubiquitin-conjugating enzyme E2H
Gene symbol UBE2H
Other names/aliases E2-20K
GID3
UBC8
UBCH
UBCH2
Species Homo sapiens
 Database cross references - UBE2H
ExoCarta ExoCarta_7328
Entrez Gene 7328
HGNC 12484
MIM 601082
UniProt P62256  
 UBE2H identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for UBE2H
Molecular Function
    ligase activity GO:0016874 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IBA
    ATP binding GO:0005524 IEA
Biological Process
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K11-linked ubiquitination GO:0070979 IDA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified UBE2H in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for UBE2H
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2AA3 8337
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
2 TRIAD3  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which UBE2H is involved
PathwayEvidenceSource
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome





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