Gene description for Plce1
Gene name phospholipase C, epsilon 1
Gene symbol Plce1
Other names/aliases 4933403A21Rik
PLCepsilon
Plce
mKIAA1516
Species Mus musculus
 Database cross references - Plce1
ExoCarta ExoCarta_74055
Entrez Gene 74055
UniProt Q8K4S1  
 Plce1 identified in exosomes derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Mast cells 17486113    
 Gene ontology annotations for Plce1
Molecular Function
    phosphoric diester hydrolase activity GO:0008081 IEA
    hydrolase activity GO:0016787 IEA
    phospholipase C activity GO:0004629 ISO
    Ras GTPase binding GO:0017016 ISO
    phosphatidylinositol phospholipase C activity GO:0004435 ISO
    signal transducer activity GO:0004871 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IEA
    enzyme binding GO:0019899 ISO
Biological Process
    regulation of protein kinase activity GO:0045859 ISO
    lipid catabolic process GO:0016042 IEA
    regulation of G-protein coupled receptor protein signaling pathway GO:0008277 ISO
    intracellular signal transduction GO:0035556 IEA
    glomerulus development GO:0032835 ISO
    Ras protein signal transduction GO:0007265 ISO
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    lipid metabolic process GO:0006629 IEA
    phospholipase C-activating G-protein coupled receptor signaling pathway GO:0007200 ISO
    regulation of Ras protein signal transduction GO:0046578 ISO
    activation of MAPK activity GO:0000187 ISO
    signal transduction GO:0007165 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    cytosol GO:0005829 ISO
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Plce1 in exosomes
1
Experiment ID 89
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Plce1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Plce1 is involved
PathwayEvidenceSource
Synthesis of IP3 and IP4 in the cytosol IEA Reactome





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