Gene description for Pus7
Gene name pseudouridylate synthase 7 homolog (S. cerevisiae)
Gene symbol Pus7
Other names/aliases C330017I15Rik
Species Mus musculus
 Database cross references - Pus7
ExoCarta ExoCarta_78697
Vesiclepedia VP_78697
Entrez Gene 78697
 Pus7 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Pus7
Molecular Function
    RNA binding GO:0003723 IEA
    pseudouridine synthase activity GO:0009982 IBA
    pseudouridine synthase activity GO:0009982 ISO
    pseudouridine synthase activity GO:0009982 ISS
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    tRNA pseudouridine(13) synthase activity GO:0160150 IEA
    tRNA pseudouridine(13) synthase activity GO:0160150 ISO
Biological Process
    pseudouridine synthesis GO:0001522 IBA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    negative regulation of translation GO:0017148 ISO
    negative regulation of translation GO:0017148 ISS
    tRNA pseudouridine synthesis GO:0031119 ISO
    tRNA pseudouridine synthesis GO:0031119 ISS
    regulation of hematopoietic stem cell differentiation GO:1902036 ISO
    regulation of hematopoietic stem cell differentiation GO:1902036 ISS
    mRNA pseudouridine synthesis GO:1990481 ISO
    mRNA pseudouridine synthesis GO:1990481 ISS
    regulation of mesoderm development GO:2000380 ISO
    regulation of mesoderm development GO:2000380 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
 Experiment description of studies that identified Pus7 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Pus7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Pus7 is involved
No pathways found





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