Gene description for Akr1a1
Gene name aldo-keto reductase family 1, member A1 (aldehyde reductase)
Gene symbol Akr1a1
Other names/aliases Akr1a4
Species Rattus norvegicus
 Database cross references - Akr1a1
ExoCarta ExoCarta_78959
Vesiclepedia VP_78959
Entrez Gene 78959
UniProt P51635  
 Akr1a1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Akr1a1
Molecular Function
    aldose reductase (NADPH) activity GO:0004032 IBA
    aldose reductase (NADPH) activity GO:0004032 ISO
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 ISO
    all-trans-retinol dehydrogenase (NAD+) activity GO:0004745 ISS
    alcohol dehydrogenase (NADP+) activity GO:0008106 IEA
    allyl-alcohol dehydrogenase activity GO:0047655 IEA
    L-glucuronate reductase activity GO:0047939 IDA
    L-glucuronate reductase activity GO:0047939 ISO
    glucuronolactone reductase activity GO:0047941 IDA
    glucuronolactone reductase activity GO:0047941 ISO
    glycerol dehydrogenase (NADP+) activity GO:0047956 IEA
    S-nitrosoglutathione reductase (NADH) activity GO:0080007 ISO
    S-nitrosoglutathione reductase (NADPH) activity GO:0160163 IEA
    methylglyoxal reductase (NADPH) (acetol producing) activity GO:1990002 IEA
Biological Process
    lipid metabolic process GO:0006629 IEA
    D-glucuronate catabolic process to D-xylulose 5-phosphate GO:0019640 ISO
    L-ascorbic acid biosynthetic process GO:0019853 IMP
    L-ascorbic acid biosynthetic process GO:0019853 ISO
    L-ascorbic acid biosynthetic process GO:0019853 ISO
    D-glucuronate catabolic process GO:0042840 IMP
    D-glucuronate catabolic process GO:0042840 ISO
    negative regulation of apoptotic process GO:0043066 IDA
    daunorubicin metabolic process GO:0044597 ISO
    doxorubicin metabolic process GO:0044598 ISO
    aldehyde catabolic process GO:0046185 IEA
    aldehyde catabolic process GO:0046185 ISO
    cellular detoxification of aldehyde GO:0110095 IDA
    cellular detoxification of aldehyde GO:0110095 ISO
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    apical plasma membrane GO:0016324 ISS
    synapse GO:0045202 ISO
 Experiment description of studies that identified Akr1a1 in exosomes
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Akr1a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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