Gene description for Arf4
Gene name ADP-ribosylation factor 4
Gene symbol Arf4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arf4
ExoCarta ExoCarta_79120
Entrez Gene 79120
UniProt P61751  
 Arf4 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Arf4
Molecular Function
    epidermal growth factor receptor binding GO:0005154 ISO
    GTP binding GO:0005525 ISO
Biological Process
    response to axon injury GO:0048678 IEP
    brain development GO:0007420 IEP
    small GTPase mediated signal transduction GO:0007264 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    vesicle-mediated transport GO:0016192 IEA
    protein transport GO:0015031 IEA
    protein localization to cilium GO:0061512 ISO
    cell migration GO:0016477 ISO
    epidermal growth factor receptor signaling pathway GO:0007173 IDA
    learning GO:0007612 ISO
    dendritic spine development GO:0060996 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 ISO
    protein ADP-ribosylation GO:0006471 ISO
    regulation of reactive oxygen species metabolic process GO:2000377 ISO
    activation of phospholipase D activity GO:0031584 ISO
    apical protein localization GO:0045176 ISO
    negative regulation of apoptotic process GO:0043066 ISO
Subcellular Localization
    cellular_component GO:0005575 ND
    Golgi apparatus GO:0005794 ISO
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    ruffle membrane GO:0032587 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Arf4 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Arf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Arf4 is involved
PathwayEvidenceSource
VxPx cargo-targeting to cilium IEA Reactome





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