Gene description for Arf4
Gene name ADP-ribosylation factor 4
Gene symbol Arf4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arf4
ExoCarta ExoCarta_79120
Vesiclepedia VP_79120
Entrez Gene 79120
UniProt P61751  
 Arf4 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Arf4
Molecular Function
    GTPase activity GO:0003924 IEA
    epidermal growth factor receptor binding GO:0005154 IEA
    epidermal growth factor receptor binding GO:0005154 ISO
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    NAD+-protein-arginine ADP-ribosyltransferase activity GO:0106274 IEA
    NAD+-protein-arginine ADP-ribosyltransferase activity GO:0106274 ISO
    phospholipase D activator activity GO:1990583 IEA
    phospholipase D activator activity GO:1990583 ISO
Biological Process
    intracellular protein transport GO:0006886 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IEA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 ISO
    epidermal growth factor receptor signaling pathway GO:0007173 IDA
    learning GO:0007612 IEA
    learning GO:0007612 ISO
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
    apical protein localization GO:0045176 IEA
    apical protein localization GO:0045176 ISO
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 IEA
    establishment or maintenance of epithelial cell apical/basal polarity GO:0045197 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    response to axon injury GO:0048678 IEP
    regulation of synapse organization GO:0050807 ISO
    dendritic spine development GO:0060996 IEA
    dendritic spine development GO:0060996 ISO
    protein localization to cilium GO:0061512 IEA
    protein localization to cilium GO:0061512 ISO
    regulation of postsynapse organization GO:0099175 IEA
    regulation of postsynapse organization GO:0099175 ISO
    regulation of cilium assembly GO:1902017 IEA
    regulation of cilium assembly GO:1902017 ISO
    regulation of cilium assembly GO:1902017 ISS
    regulation of reactive oxygen species metabolic process GO:2000377 IEA
    regulation of reactive oxygen species metabolic process GO:2000377 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    Golgi membrane GO:0000139 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    ruffle membrane GO:0032587 IEA
    ruffle membrane GO:0032587 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Arf4 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Arf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Fbxo21  
Affinity Capture-MS Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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