Gene description for Anxa4
Gene name annexin A4
Gene symbol Anxa4
Other names/aliases Anx4
Species Rattus norvegicus
 Database cross references - Anxa4
ExoCarta ExoCarta_79124
Vesiclepedia VP_79124
Entrez Gene 79124
 Anxa4 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
 Gene ontology annotations for Anxa4
Molecular Function
    phosphatidylserine binding GO:0001786 IBA
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    calcium-dependent phospholipid binding GO:0005544 IBA
    calcium-dependent phospholipid binding GO:0005544 IEA
    calcium-dependent phospholipid binding GO:0005544 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    calcium-dependent protein binding GO:0048306 IEA
    calcium-dependent protein binding GO:0048306 ISO
    NF-kappaB binding GO:0051059 IEA
    NF-kappaB binding GO:0051059 ISO
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    exocytosis GO:0006887 NAS
    Notch signaling pathway GO:0007219 IEA
    Notch signaling pathway GO:0007219 ISO
    epithelial cell differentiation GO:0030855 IEA
    epithelial cell differentiation GO:0030855 ISO
    negative regulation of NF-kappaB transcription factor activity GO:0032088 ISO
    negative regulation of interleukin-8 production GO:0032717 IEA
    negative regulation of interleukin-8 production GO:0032717 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    vesicle membrane GO:0012506 IBA
    vesicle membrane GO:0012506 ISO
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    zymogen granule membrane GO:0042589 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Anxa4 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Anxa4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Anxa4 is involved
No pathways found





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