Gene description for Gk
Gene name glycerol kinase
Gene symbol Gk
Other names/aliases ASTP
Gyk
Species Rattus norvegicus
 Database cross references - Gk
ExoCarta ExoCarta_79223
Vesiclepedia VP_79223
Entrez Gene 79223
UniProt Q63060  
 Gk identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gk
Molecular Function
    glycerol kinase activity GO:0004370 IBA
    glycerol kinase activity GO:0004370 IEA
    glycerol kinase activity GO:0004370 ISO
    glycerol kinase activity GO:0004370 ISS
    ATP binding GO:0005524 IEA
    histone binding GO:0042393 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    glycerol metabolic process GO:0006071 IBA
    glycerol metabolic process GO:0006071 ISO
    glycerol metabolic process GO:0006071 ISO
    glycerol-3-phosphate metabolic process GO:0006072 IEA
    triglyceride metabolic process GO:0006641 IBA
    triglyceride metabolic process GO:0006641 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    regulation of fatty acid metabolic process GO:0019217 ISO
    triglyceride biosynthetic process GO:0019432 ISO
    glycerol catabolic process GO:0019563 IEA
    glucose homeostasis GO:0042593 ISO
    response to ethanol GO:0045471 IDA
    glycerol-3-phosphate biosynthetic process GO:0046167 IBA
    glycerol-3-phosphate biosynthetic process GO:0046167 ISO
    glycerol-3-phosphate biosynthetic process GO:0046167 ISS
    response to norepinephrine GO:0071873 IEP
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
 Experiment description of studies that identified Gk in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gk is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Triglyceride biosynthesis IEA Reactome
Triglyceride metabolism IEA Reactome





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