Gene description for Hsd17b4
Gene name hydroxysteroid (17-beta) dehydrogenase 4
Gene symbol Hsd17b4
Other names/aliases MPF-2
Species Rattus norvegicus
 Database cross references - Hsd17b4
ExoCarta ExoCarta_79244
Vesiclepedia VP_79244
Entrez Gene 79244
UniProt P97852  
 Hsd17b4 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hsd17b4
Molecular Function
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IBA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 IEA
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 ISO
    3-hydroxyacyl-CoA dehydrogenase activity GO:0003857 ISS
    enoyl-CoA hydratase activity GO:0004300 IBA
    enoyl-CoA hydratase activity GO:0004300 ISO
    enoyl-CoA hydratase activity GO:0004300 ISS
    estradiol 17-beta-dehydrogenase [NAD(P)+] activity GO:0004303 IDA
    estradiol 17-beta-dehydrogenase [NAD(P)+] activity GO:0004303 IEA
    estradiol 17-beta-dehydrogenase [NAD(P)+] activity GO:0004303 ISO
    oxidoreductase activity GO:0016491 IEA
    lyase activity GO:0016829 IEA
    isomerase activity GO:0016853 IEA
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 IDA
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 IEA
    3-hydroxyacyl-CoA dehydratase activity GO:0018812 ISO
    testosterone dehydrogenase [NAD(P)+] activity GO:0030283 TAS
    3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity GO:0033989 IEA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    17-beta-hydroxysteroid dehydrogenase (NAD+) activity GO:0044594 IBA
    17-beta-hydroxysteroid dehydrogenase (NAD+) activity GO:0044594 IDA
    (3R)-hydroxyacyl-CoA dehydrogenase (NAD+) activity GO:0106386 IEA
Biological Process
    very long-chain fatty acid metabolic process GO:0000038 IEA
    very long-chain fatty acid metabolic process GO:0000038 ISO
    fatty acid beta-oxidation GO:0006635 IBA
    fatty acid beta-oxidation GO:0006635 IEA
    fatty acid beta-oxidation GO:0006635 ISO
    fatty acid beta-oxidation GO:0006635 ISO
    fatty acid beta-oxidation GO:0006635 ISS
    cholesterol metabolic process GO:0008203 NAS
    androgen metabolic process GO:0008209 IEA
    androgen metabolic process GO:0008209 ISO
    estrogen metabolic process GO:0008210 IEA
    estrogen metabolic process GO:0008210 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    response to organic cyclic compound GO:0014070 IEP
    very long-chain fatty-acyl-CoA metabolic process GO:0036111 IEA
    very long-chain fatty-acyl-CoA metabolic process GO:0036111 ISO
    medium-chain fatty-acyl-CoA metabolic process GO:0036112 IEA
    medium-chain fatty-acyl-CoA metabolic process GO:0036112 ISO
    response to steroid hormone GO:0048545 IEP
    Sertoli cell development GO:0060009 IEA
    Sertoli cell development GO:0060009 ISO
    cellular response to organic cyclic compound GO:0071407 IEP
Subcellular Localization
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 IEA
    peroxisome GO:0005777 ISO
    membrane GO:0016020 IEA
 Experiment description of studies that identified Hsd17b4 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hsd17b4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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