Gene description for Myh10
Gene name myosin, heavy chain 10, non-muscle
Gene symbol Myh10
Other names/aliases MCH-B
NMMHC-B
SMemb
Species Rattus norvegicus
 Database cross references - Myh10
ExoCarta ExoCarta_79433
Entrez Gene 79433
UniProt Q9JLT0  
 Myh10 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Myh10
Molecular Function
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    motor activity GO:0003774 IEA
    actin-dependent ATPase activity GO:0030898 ISO
    ATP binding GO:0005524 ISO
    microfilament motor activity GO:0000146 ISO
    actin filament binding GO:0051015 ISO
    ADP binding GO:0043531 ISO
Biological Process
    cerebellar Purkinje cell layer development GO:0021680 ISO
    adult heart development GO:0007512 ISO
    metabolic process GO:0008152 ISO
    actin filament-based movement GO:0030048 ISO
    nuclear migration GO:0007097 ISO
    exocytosis GO:0006887 ISO
    actomyosin structure organization GO:0031032 ISO
    substrate-dependent cell migration, cell extension GO:0006930 ISO
    axonogenesis GO:0007409 ISO
    ventricular cardiac muscle cell development GO:0055015 ISO
    in utero embryonic development GO:0001701 ISO
    fourth ventricle development GO:0021592 ISO
    axon guidance GO:0007411 ISO
    cell adhesion GO:0007155 IEA
    lateral ventricle development GO:0021670 ISO
    neuromuscular process controlling balance GO:0050885 ISO
    neuron projection development GO:0031175 ISO
    regulation of cell shape GO:0008360 ISO
    actin cytoskeleton organization GO:0030036 ISO
    third ventricle development GO:0021678 ISO
    retina development in camera-type eye GO:0060041 ISO
    cell proliferation GO:0008283 ISO
    cardiac myofibril assembly GO:0055003 ISO
    brain development GO:0007420 ISO
    myofibril assembly GO:0030239 ISO
    plasma membrane repair GO:0001778 ISO
    neuron migration GO:0001764 ISO
    mitotic cytokinesis GO:0000281 ISO
Subcellular Localization
    spindle GO:0005819 ISO
    cytoplasm GO:0005737 ISO
    myosin II complex GO:0016460 ISO
    myosin complex GO:0016459 ISO
    actomyosin GO:0042641 ISO
    mitochondrion GO:0005739 ISO
    cell cortex GO:0005938 ISO
    cleavage furrow GO:0032154 ISO
    stress fiber GO:0001725 ISO
    midbody GO:0030496 ISO
    neuronal cell body GO:0043025 ISO
    neuromuscular junction GO:0031594 ISO
    plasma membrane GO:0005886 ISO
    actin cytoskeleton GO:0015629 ISO
    nucleus GO:0005634 ISO
    axon GO:0030424 ISO
    myosin II filament GO:0097513 ISO
    extracellular exosome GO:0070062 ISO
    lamellipodium GO:0030027 IEA
    growth cone GO:0030426 ISO
    dendritic spine GO:0043197 ISO
    brush border GO:0005903 ISO
    neuron projection GO:0043005 ISO
 Experiment description of studies that identified Myh10 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Myh10
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Myh10 is involved
No pathways found





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