Gene description for Sec61a1
Gene name Sec61 alpha 1 subunit (S. cerevisiae)
Gene symbol Sec61a1
Other names/aliases Sec61a
Species Rattus norvegicus
 Database cross references - Sec61a1
ExoCarta ExoCarta_80843
Vesiclepedia VP_80843
Entrez Gene 80843
UniProt P61621  
 Sec61a1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Sec61a1
Molecular Function
    signal sequence binding GO:0005048 IBA
    calcium channel activity GO:0005262 IEA
    calcium channel activity GO:0005262 ISO
    calcium channel activity GO:0005262 ISS
    protein transmembrane transporter activity GO:0008320 IBA
    transmembrane transporter activity GO:0022857 ISO
    ribosome binding GO:0043022 IBA
    ribosome binding GO:0043022 IEA
    ribosome binding GO:0043022 ISO
    ribosome binding GO:0043022 ISS
Biological Process
    cotranslational protein targeting to membrane GO:0006613 ISO
    cotranslational protein targeting to membrane GO:0006613 ISS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 IEA
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 ISO
    SRP-dependent cotranslational protein targeting to membrane, translocation GO:0006616 IBA
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 ISO
    endoplasmic reticulum organization GO:0007029 ISO
    protein transport GO:0015031 IEA
    post-translational protein targeting to membrane, translocation GO:0031204 IBA
    post-translational protein targeting to membrane, translocation GO:0031204 IEA
    post-translational protein targeting to membrane, translocation GO:0031204 ISO
    post-translational protein targeting to membrane, translocation GO:0031204 ISS
    response to type II interferon GO:0034341 IEP
    pronephric nephron development GO:0039019 IEA
    pronephric nephron development GO:0039019 ISO
    pronephric nephron development GO:0039019 ISS
    protein targeting to ER GO:0045047 ISO
    protein insertion into ER membrane GO:0045048 IEA
    protein insertion into ER membrane GO:0045048 ISO
    protein insertion into ER membrane GO:0045048 ISS
    calcium ion transmembrane transport GO:0070588 IEA
Subcellular Localization
    Sec61 translocon complex GO:0005784 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    membrane GO:0016020 ISO
 Experiment description of studies that identified Sec61a1 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sec61a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sec61a1 is involved
No pathways found





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