Gene description for Dnajb1
Gene name DnaJ (Hsp40) homolog, subfamily B, member 1
Gene symbol Dnajb1
Other names/aliases 0610007I11Rik
DjB1
HSPF1
Hdj1
Hsp40
Species Mus musculus
 Database cross references - Dnajb1
ExoCarta ExoCarta_81489
Vesiclepedia VP_81489
Entrez Gene 81489
UniProt Q9QYJ3  
 Dnajb1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dnajb1
Molecular Function
    ATPase activator activity GO:0001671 ISO
    ATPase activator activity GO:0001671 ISS
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 ISO
    transcription corepressor activity GO:0003714 ISS
    Hsp70 protein binding GO:0030544 IBA
    Hsp70 protein binding GO:0030544 IEA
    Hsp70 protein binding GO:0030544 ISO
    protein folding chaperone GO:0044183 IDA
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    unfolded protein binding GO:0051082 ISO
    protein-folding chaperone binding GO:0051087 ISO
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
    transcription regulator inhibitor activity GO:0140416 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    protein folding GO:0006457 IDA
    positive regulation of ATP-dependent activity GO:0032781 ISO
    cellular response to heat GO:0034605 IEA
    cellular response to heat GO:0034605 ISO
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
    chaperone cofactor-dependent protein refolding GO:0051085 IGI
    chaperone cofactor-dependent protein refolding GO:0051085 ISO
    negative regulation of inclusion body assembly GO:0090084 IEA
    negative regulation of inclusion body assembly GO:0090084 ISO
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    postsynaptic density GO:0014069 ISO
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 ISO
    sperm head GO:0061827 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Dnajb1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dnajb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-RNA Mus musculus
2 Htt  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Atg16l1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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