Gene description for Bhmt
Gene name betaine-homocysteine S-methyltransferase
Gene symbol Bhmt
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Bhmt
ExoCarta ExoCarta_81508
Vesiclepedia VP_81508
Entrez Gene 81508
UniProt O09171  
 Bhmt identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Bhmt
Molecular Function
    methyltransferase activity GO:0008168 IDA
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    identical protein binding GO:0042802 IDA
    protein-containing complex binding GO:0044877 IDA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IBA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IDA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IEA
    betaine-homocysteine S-methyltransferase activity GO:0047150 IMP
    betaine-homocysteine S-methyltransferase activity GO:0047150 ISO
Biological Process
    protein methylation GO:0006479 IDA
    amino-acid betaine metabolic process GO:0006577 IEA
    amino-acid betaine metabolic process GO:0006577 ISO
    amino-acid betaine catabolic process GO:0006579 IEA
    methionine biosynthetic process GO:0009086 IDA
    methionine biosynthetic process GO:0009086 ISO
    methylation GO:0032259 IEA
    S-adenosylmethionine metabolic process GO:0046500 ISO
    'de novo' L-methionine biosynthetic process GO:0071266 IEA
    'de novo' L-methionine biosynthetic process GO:0071266 ISO
    L-methionine salvage GO:0071267 IBA
    L-methionine salvage GO:0071267 IEA
    L-methionine salvage GO:0071267 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IDA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Bhmt in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Bhmt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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