Gene description for Lat
Gene name linker for activation of T cells
Gene symbol Lat
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Lat
ExoCarta ExoCarta_81511
Entrez Gene 81511
UniProt O70601  
 Lat identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Lat
Molecular Function
    SH3/SH2 adaptor activity GO:0005070 ISO
    protein kinase binding GO:0019901 ISO
Biological Process
    mast cell degranulation GO:0043303 IEA
    integrin-mediated signaling pathway GO:0007229 ISO
    gene expression GO:0010467 ISO
    positive regulation of protein kinase activity GO:0045860 ISO
    immune response GO:0006955 ISO
    positive regulation of signal transduction GO:0009967 ISO
    intracellular signal transduction GO:0035556 ISO
    regulation of T cell activation GO:0050863 ISO
    inflammatory response GO:0006954 ISO
    homeostasis of number of cells GO:0048872 ISO
    calcium-mediated signaling GO:0019722 ISO
    Ras protein signal transduction GO:0007265 ISO
    lymphocyte homeostasis GO:0002260 ISO
Subcellular Localization
    integral component of membrane GO:0016021 ISO
    mast cell granule GO:0042629 IEA
    cell-cell junction GO:0005911 ISO
    COP9 signalosome GO:0008180 ISS
    immunological synapse GO:0001772 ISO
 Experiment description of studies that identified Lat in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Lat
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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