Gene description for Msn
Gene name moesin
Gene symbol Msn
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Msn
ExoCarta ExoCarta_81521
Vesiclepedia VP_81521
Entrez Gene 81521
UniProt O35763  
 Msn identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Msn
Molecular Function
    double-stranded RNA binding GO:0003725 IEA
    double-stranded RNA binding GO:0003725 ISO
    actin binding GO:0003779 IBA
    actin binding GO:0003779 IDA
    actin binding GO:0003779 IEA
    signaling receptor binding GO:0005102 IEA
    signaling receptor binding GO:0005102 ISO
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    protein-macromolecule adaptor activity GO:0030674 TAS
    cell adhesion molecule binding GO:0050839 IBA
    cell adhesion molecule binding GO:0050839 IEA
    cell adhesion molecule binding GO:0050839 ISO
Biological Process
    immunological synapse formation GO:0001771 IEA
    immunological synapse formation GO:0001771 ISO
    immunological synapse formation GO:0001771 ISS
    leukocyte cell-cell adhesion GO:0007159 ISO
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 IEA
    regulation of cell shape GO:0008360 ISO
    regulation of cell size GO:0008361 IEA
    regulation of cell size GO:0008361 ISO
    regulation of cell size GO:0008361 ISO
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    gland morphogenesis GO:0022612 IEA
    gland morphogenesis GO:0022612 ISO
    membrane to membrane docking GO:0022614 IEA
    membrane to membrane docking GO:0022614 ISO
    T cell proliferation GO:0042098 IEA
    T cell proliferation GO:0042098 ISO
    T cell proliferation GO:0042098 ISS
    establishment of epithelial cell apical/basal polarity GO:0045198 IEA
    establishment of epithelial cell apical/basal polarity GO:0045198 ISO
    positive regulation of protein catabolic process GO:0045732 IEA
    positive regulation of protein catabolic process GO:0045732 ISO
    leukocyte migration GO:0050900 ISO
    establishment of endothelial barrier GO:0061028 IEA
    establishment of endothelial barrier GO:0061028 ISO
    T cell aggregation GO:0070489 IEA
    T cell aggregation GO:0070489 ISO
    T cell aggregation GO:0070489 ISS
    cellular response to testosterone stimulus GO:0071394 IEA
    cellular response to testosterone stimulus GO:0071394 ISO
    positive regulation of podosome assembly GO:0071803 IEA
    positive regulation of podosome assembly GO:0071803 ISO
    T cell migration GO:0072678 IEA
    T cell migration GO:0072678 ISO
    T cell migration GO:0072678 ISS
    regulation of organelle assembly GO:1902115 IBA
    regulation of organelle assembly GO:1902115 ISO
    positive regulation of protein localization to early endosome GO:1902966 IBA
    positive regulation of protein localization to early endosome GO:1902966 IEA
    positive regulation of protein localization to early endosome GO:1902966 ISO
    regulation of lymphocyte migration GO:2000401 IEA
    regulation of lymphocyte migration GO:2000401 ISO
    positive regulation of early endosome to late endosome transport GO:2000643 IBA
    positive regulation of early endosome to late endosome transport GO:2000643 IEA
    positive regulation of early endosome to late endosome transport GO:2000643 ISO
Subcellular Localization
    uropod GO:0001931 IEA
    uropod GO:0001931 ISO
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 IBA
    microvillus GO:0005902 IEA
    microvillus GO:0005902 ISO
    microvillus GO:0005902 ISS
    adherens junction GO:0005912 IBA
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    cytoplasmic side of plasma membrane GO:0009898 IDA
    cell surface GO:0009986 IDA
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    basolateral plasma membrane GO:0016323 IEA
    basolateral plasma membrane GO:0016323 ISO
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    filopodium GO:0030175 IBA
    filopodium GO:0030175 IEA
    filopodium GO:0030175 ISO
    T-tubule GO:0030315 IDA
    pseudopodium GO:0031143 IEA
    pseudopodium GO:0031143 ISO
    filopodium membrane GO:0031527 IDA
    microvillus membrane GO:0031528 IEA
    apical part of cell GO:0045177 IBA
    apical part of cell GO:0045177 ISO
    perinuclear region of cytoplasm GO:0048471 IEA
    perinuclear region of cytoplasm GO:0048471 ISO
    cell tip GO:0051286 IDA
    cell periphery GO:0071944 ISO
 Experiment description of studies that identified Msn in exosomes
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Msn
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sumo3  
Affinity Capture-MS Rattus norvegicus
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