Gene description for Chia1
Gene name chitinase, acidic 1
Gene symbol Chia1
Other names/aliases 2200003E03Rik
AMCase
Chia
YNL
Species Mus musculus
 Database cross references - Chia1
ExoCarta ExoCarta_81600
Vesiclepedia VP_81600
Entrez Gene 81600
UniProt Q91XA9  
 Chia1 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Chia1
Molecular Function
    chitinase activity GO:0004568 IBA
    chitinase activity GO:0004568 IDA
    chitinase activity GO:0004568 ISO
    chitinase activity GO:0004568 ISS
    chitin binding GO:0008061 IBA
    chitin binding GO:0008061 IDA
    endochitinase activity GO:0008843 IEA
    kinase binding GO:0019900 ISO
Biological Process
    polysaccharide catabolic process GO:0000272 IEA
    immune system process GO:0002376 IEA
    production of molecular mediator involved in inflammatory response GO:0002532 ISO
    production of molecular mediator involved in inflammatory response GO:0002532 ISS
    chitin catabolic process GO:0006032 IBA
    chitin catabolic process GO:0006032 IDA
    chitin catabolic process GO:0006032 ISO
    chitin catabolic process GO:0006032 ISS
    apoptotic process GO:0006915 IEA
    positive regulation of chemokine production GO:0032722 ISO
    positive regulation of chemokine production GO:0032722 ISS
Subcellular Localization
    extracellular region GO:0005576 IBA
    extracellular region GO:0005576 ISO
    extracellular space GO:0005615 IC
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Chia1 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Chia1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Chia1 is involved
PathwayEvidenceSource
Digestion IEA Reactome
Digestion and absorption IEA Reactome
Digestion of dietary carbohydrate IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here