Gene description for Abat
Gene name 4-aminobutyrate aminotransferase
Gene symbol Abat
Other names/aliases Gabat
beta-AlaAT
Species Rattus norvegicus
 Database cross references - Abat
ExoCarta ExoCarta_81632
Entrez Gene 81632
UniProt P50554  
 Abat identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Abat
Molecular Function
    iron-sulfur cluster binding GO:0051536 IEA
    succinate-semialdehyde dehydrogenase binding GO:0032145 ISS
    (S)-3-amino-2-methylpropionate transaminase activity GO:0047298 IEA
    4-aminobutyrate transaminase activity GO:0003867 ISS
    protein homodimerization activity GO:0042803 ISS
    metal ion binding GO:0046872 IEA
    pyridoxal phosphate binding GO:0030170 ISS
Biological Process
    locomotory behavior GO:0007626 IMP
    response to iron ion GO:0010039 IDA
    gamma-aminobutyric acid biosynthetic process GO:0009449 IMP
    copulation GO:0007620 IMP
    response to drug GO:0042493 IDA
    positive regulation of insulin secretion GO:0032024 IMP
    behavioral response to cocaine GO:0048148 ISS
    negative regulation of blood pressure GO:0045776 IMP
    response to nicotine GO:0035094 IMP
    response to ethanol GO:0045471 IDA
    neurotransmitter catabolic process GO:0042135 IC
    response to hypoxia GO:0001666 IMP
    positive regulation of heat generation GO:0031652 IMP
    gamma-aminobutyric acid catabolic process GO:0009450 IC
    response to cocaine GO:0042220 IMP
Subcellular Localization
    mitochondrial matrix GO:0005759 IEA
    4-aminobutyrate transaminase complex GO:0032144 ISS
    mitochondrion GO:0005739 ISS
    neuron projection GO:0043005 IDA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Abat in exosomes
1
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 92
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
3
Experiment ID 93
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Abat
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Abat is involved
PathwayEvidenceSource
Degradation of GABA IEA Reactome





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