Gene description for Gna12
Gene name guanine nucleotide binding protein (G protein) alpha 12
Gene symbol Gna12
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gna12
ExoCarta ExoCarta_81663
Vesiclepedia VP_81663
Entrez Gene 81663
UniProt Q63210  
 Gna12 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gna12
Molecular Function
    GTPase activity GO:0003924 IBA
    G protein activity GO:0003925 ISO
    GTP binding GO:0005525 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    D5 dopamine receptor binding GO:0031752 IBA
    D5 dopamine receptor binding GO:0031752 IPI
    metal ion binding GO:0046872 IEA
    protein phosphatase 2A binding GO:0051721 ISO
    protein phosphatase activator activity GO:0072542 IEA
    protein phosphatase activator activity GO:0072542 ISO
Biological Process
    in utero embryonic development GO:0001701 ISO
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-modulating G protein-coupled receptor signaling pathway GO:0007188 IBA
    G protein-coupled dopamine receptor signaling pathway GO:0007212 TAS
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 ISO
    regulation of blood pressure GO:0008217 ISO
    regulation of cell shape GO:0008360 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    regulation of fibroblast migration GO:0010762 ISO
    regulation of fibroblast migration GO:0010762 ISS
    cell differentiation GO:0030154 ISO
    regulation of TOR signaling GO:0032006 ISO
    regulation of TOR signaling GO:0032006 ISS
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISO
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISS
    intracellular signal transduction GO:0035556 ISO
    embryonic digit morphogenesis GO:0042733 ISO
    negative regulation of vascular associated smooth muscle cell proliferation GO:1904706 IMP
    negative regulation of vascular associated smooth muscle cell migration GO:1904753 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 TAS
    lateral plasma membrane GO:0016328 ISO
    brush border membrane GO:0031526 IBA
    brush border membrane GO:0031526 IDA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 ISO
 Experiment description of studies that identified Gna12 in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gna12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gna12 is involved
PathwayEvidenceSource
G alpha (12/13) signalling events IEA Reactome
GPCR downstream signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome





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