Gene description for Gna12
Gene name guanine nucleotide binding protein (G protein) alpha 12
Gene symbol Gna12
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gna12
ExoCarta ExoCarta_81663
Entrez Gene 81663
UniProt Q63210  
 Gna12 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gna12
Molecular Function
    signal transducer activity GO:0004871 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    GTPase activity GO:0003924 IBA
    D5 dopamine receptor binding GO:0031752 IPI
    metal ion binding GO:0046872 IEA
    GTP binding GO:0005525 IEA
Biological Process
    in utero embryonic development GO:0001701 ISO
    cell differentiation GO:0030154 ISO
    dopamine receptor signaling pathway GO:0007212 TAS
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISS
    intracellular signal transduction GO:0035556 ISO
    Rho protein signal transduction GO:0007266 ISO
    regulation of cell shape GO:0008360 ISO
    adenylate cyclase-modulating G-protein coupled receptor signaling pathway GO:0007188 IBA
    regulation of fibroblast migration GO:0010762 ISS
    metabolic process GO:0008152 IEA
    response to drug GO:0042493 IEP
    embryonic digit morphogenesis GO:0042733 ISO
    regulation of TOR signaling GO:0032006 ISS
Subcellular Localization
    heterotrimeric G-protein complex GO:0005834 TAS
    brush border membrane GO:0031526 IDA
    focal adhesion GO:0005925 ISO
    intracellular GO:0005622 ISO
 Experiment description of studies that identified Gna12 in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gna12
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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