Gene description for Gnaq
Gene name guanine nucleotide binding protein (G protein), q polypeptide
Gene symbol Gnaq
Other names/aliases Galphaq
Species Rattus norvegicus
 Database cross references - Gnaq
ExoCarta ExoCarta_81666
Entrez Gene 81666
UniProt P82471  
 Gnaq identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Gnaq
Molecular Function
    GTPase activator activity GO:0005096 ISO
    GTP binding GO:0005525 IEA
    protein complex binding GO:0032403 IPI
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    GTPase activity GO:0003924 IEA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    type 2A serotonin receptor binding GO:0031826 IMP
    G-protein coupled receptor binding GO:0001664 IEA
    signal transducer activity GO:0004871 IEA
Biological Process
    glutamate receptor signaling pathway GO:0007215 ISO
    protein stabilization GO:0050821 ISO
    metabolic process GO:0008152 IEA
    phospholipase C-activating G-protein coupled receptor signaling pathway GO:0007200 IMP
    adenylate cyclase-activating G-protein coupled receptor signaling pathway GO:0007189 ISO
    action potential GO:0001508 ISO
    post-embryonic development GO:0009791 ISO
    heart development GO:0007507 ISO
    positive regulation of smooth muscle cell proliferation GO:0048661 IMP
    positive regulation of apoptotic process GO:0043065 TAS
    negative regulation of protein kinase activity GO:0006469 ISO
    maternal behavior GO:0042711 ISO
    negative regulation of potassium ion transport GO:0043267 IMP
    embryonic digit morphogenesis GO:0042733 ISO
    neuron remodeling GO:0016322 ISO
    skeletal system development GO:0001501 ISO
    regulation of melanocyte differentiation GO:0045634 ISO
    regulation of catenin import into nucleus GO:0035412 ISO
    negative regulation of apoptotic process GO:0043066 IMP
    developmental pigmentation GO:0048066 ISO
    activation of phospholipase C activity GO:0007202 IMP
    phospholipase C-activating dopamine receptor signaling pathway GO:0060158 ISO
    forebrain neuron development GO:0021884 ISO
    G-protein coupled receptor signaling pathway GO:0007186 ISO
    positive regulation of GTPase activity GO:0043547 ISO
Subcellular Localization
    protein complex GO:0043234 IDA
    plasma membrane GO:0005886 IDA
    cytosol GO:0005829 IDA
    caveola GO:0005901 IDA
    lysosomal membrane GO:0005765 ISO
    heterotrimeric G-protein complex GO:0005834 TAS
    extracellular exosome GO:0070062 ISO
    membrane GO:0016020 ISO
    nuclear membrane GO:0031965 IEA
 Experiment description of studies that identified Gnaq in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gnaq
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gnaq is involved
No pathways found





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