Gene description for Gnaq
Gene name guanine nucleotide binding protein (G protein), q polypeptide
Gene symbol Gnaq
Other names/aliases Galphaq
Species Rattus norvegicus
 Database cross references - Gnaq
ExoCarta ExoCarta_81666
Vesiclepedia VP_81666
Entrez Gene 81666
UniProt P82471  
 Gnaq identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Gnaq
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    G protein-coupled receptor binding GO:0001664 IEA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    G protein activity GO:0003925 IEA
    G protein activity GO:0003925 ISO
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    enzyme regulator activity GO:0030234 IEA
    enzyme regulator activity GO:0030234 ISO
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    protein-containing complex binding GO:0044877 IPI
    metal ion binding GO:0046872 IEA
    alkylglycerophosphoethanolamine phosphodiesterase activity GO:0047391 IDA
Biological Process
    skeletal system development GO:0001501 IEA
    skeletal system development GO:0001501 ISO
    action potential GO:0001508 IEA
    action potential GO:0001508 ISO
    negative regulation of protein kinase activity GO:0006469 ISO
    G protein-coupled receptor signaling pathway GO:0007186 IEA
    G protein-coupled receptor signaling pathway GO:0007186 ISO
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IEA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 ISO
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IMP
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISO
    phospholipase C-activating G protein-coupled glutamate receptor signaling pathway GO:0007206 ISO
    phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway GO:0007207 IEA
    phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway GO:0007207 ISO
    phospholipase C-activating serotonin receptor signaling pathway GO:0007208 ISO
    glutamate receptor signaling pathway GO:0007215 IBA
    glutamate receptor signaling pathway GO:0007215 IEA
    glutamate receptor signaling pathway GO:0007215 ISO
    neuropeptide signaling pathway GO:0007218 ISO
    heart development GO:0007507 IEA
    heart development GO:0007507 ISO
    regulation of blood pressure GO:0008217 IEA
    regulation of blood pressure GO:0008217 ISO
    post-embryonic development GO:0009791 IEA
    post-embryonic development GO:0009791 ISO
    regulation of platelet activation GO:0010543 IEA
    regulation of platelet activation GO:0010543 ISO
    regulation of platelet activation GO:0010543 ISS
    neuron remodeling GO:0016322 IEA
    neuron remodeling GO:0016322 ISO
    forebrain neuron development GO:0021884 IEA
    forebrain neuron development GO:0021884 ISO
    positive regulation of insulin secretion GO:0032024 IEA
    positive regulation of insulin secretion GO:0032024 ISO
    response to prostaglandin E GO:0034695 ISO
    maternal behavior GO:0042711 IEA
    maternal behavior GO:0042711 ISO
    embryonic digit morphogenesis GO:0042733 IEA
    embryonic digit morphogenesis GO:0042733 ISO
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of potassium ion transport GO:0043267 IMP
    regulation of melanocyte differentiation GO:0045634 IEA
    regulation of melanocyte differentiation GO:0045634 ISO
    developmental pigmentation GO:0048066 IEA
    developmental pigmentation GO:0048066 ISO
    protein stabilization GO:0050821 ISO
    phospholipase C-activating dopamine receptor signaling pathway GO:0060158 IEA
    phospholipase C-activating dopamine receptor signaling pathway GO:0060158 ISO
    regulation of canonical Wnt signaling pathway GO:0060828 ISO
    endothelin receptor signaling pathway GO:0086100 IEA
    endothelin receptor signaling pathway GO:0086100 ISO
    ion channel modulating, G protein-coupled receptor signaling pathway GO:0099105 IEA
    ion channel modulating, G protein-coupled receptor signaling pathway GO:0099105 ISO
    cranial skeletal system development GO:1904888 IEA
    cranial skeletal system development GO:1904888 ISO
    ligand-gated ion channel signaling pathway GO:1990806 IEA
    ligand-gated ion channel signaling pathway GO:1990806 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IEA
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 IDA
    heterotrimeric G-protein complex GO:0005834 IEA
    plasma membrane GO:0005886 ISO
    caveola GO:0005901 IDA
    membrane GO:0016020 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    nuclear membrane GO:0031965 IEA
    cell body GO:0044297 IEA
    cell body GO:0044297 ISO
    synapse GO:0045202 IEA
    presynapse GO:0098793 IDA
    presynapse GO:0098793 NAS
    postsynaptic cytosol GO:0099524 ISO
 Experiment description of studies that identified Gnaq in exosomes
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gnaq
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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