Gene description for Mif
Gene name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Gene symbol Mif
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Mif
ExoCarta ExoCarta_81683
Entrez Gene 81683
UniProt P30904  
 Mif identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Mif
Molecular Function
    cytokine activity GO:0005125 ISO
    protein complex binding GO:0032403 IDA
    phenylpyruvate tautomerase activity GO:0050178 ISO
    receptor binding GO:0005102 ISO
    chemoattractant activity GO:0042056 ISO
    cytokine receptor binding GO:0005126 ISO
    dopachrome isomerase activity GO:0004167 ISS
Biological Process
    carboxylic acid metabolic process GO:0019752 ISO
    response to lipopolysaccharide GO:0032496 IEP
    hair follicle development GO:0001942 IEP
    metabolic process GO:0008152 ISS
    positive regulation of protein phosphorylation GO:0001934 ISO
    positive regulation of arachidonic acid secretion GO:0090238 ISO
    spermatogenesis GO:0007283 IMP
    cellular response to hydrogen peroxide GO:0070301 IEP
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 ISO
    positive regulation of protein kinase A signaling GO:0010739 ISO
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    cell proliferation GO:0008283 ISO
    positive regulation of myeloid leukocyte cytokine production involved in immune response GO:0061081 ISO
    response to vitamin E GO:0033197 IEP
    positive regulation of MAP kinase activity GO:0043406 ISO
    negative regulation of cell cycle arrest GO:0071157 ISO
    negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:1902166 ISO
    positive regulation of glycolytic process GO:0045821 IMP
    response to insulin GO:0032868 IEP
    cellular response to hypoxia GO:0071456 IEP
    positive regulation of potassium ion transport GO:0043268 IMP
    DNA damage response, signal transduction by p53 class mediator GO:0030330 ISO
    positive regulation of immune response GO:0050778 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of lipopolysaccharide-mediated signaling pathway GO:0031666 ISO
    response to glucocorticoid GO:0051384 IEP
    response to heat GO:0009408 IEP
    inflammatory response GO:0006954 IEA
    cell aging GO:0007569 ISO
    brain renin-angiotensin system GO:0002035 IMP
    skin development GO:0043588 IEP
    innate immune response GO:0045087 IEA
    response to drug GO:0042493 IEP
    negative regulation of mature B cell apoptotic process GO:0002906 ISO
    positive regulation of B cell proliferation GO:0030890 ISO
    positive regulation of cytokine secretion GO:0050715 ISO
    response to progesterone GO:0032570 IEP
    positive regulation of fibroblast proliferation GO:0048146 ISO
    positive regulation of prostaglandin secretion involved in immune response GO:0061078 ISO
    brain development GO:0007420 IEP
    prostaglandin biosynthetic process GO:0001516 ISO
    negative regulation of cellular protein metabolic process GO:0032269 ISO
    negative regulation of myeloid cell apoptotic process GO:0033033 ISO
    response to mechanical stimulus GO:0009612 IEP
    negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043518 ISO
    positive regulation of acute inflammatory response GO:0002675 IMP
    positive regulation of smooth muscle cell migration GO:0014911 IMP
    response to menaquinone GO:0032572 IEP
    response to inorganic substance GO:0010035 IEP
    positive regulation of chemokine (C-X-C motif) ligand 2 production GO:2000343 ISO
    drinking behavior GO:0042756 IMP
    positive regulation of glucose import GO:0046326 IMP
    positive regulation of phosphorylation GO:0042327 ISO
    aging GO:0007568 IEP
    response to hormone GO:0009725 IEP
    negative regulation of cell aging GO:0090344 ISO
    regulation of cell proliferation GO:0042127 ISO
    positive chemotaxis GO:0050918 ISO
    protein homotrimerization GO:0070207 ISO
    response to estradiol GO:0032355 IEP
    negative regulation of apoptotic process GO:0043066 ISO
    cell surface receptor signaling pathway GO:0007166 ISO
    wound healing GO:0042060 IEP
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of axon regeneration GO:0048680 IMP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    vesicle GO:0031982 ISO
    extracellular exosome GO:0070062 ISO
    myelin sheath GO:0043209 ISO
    nucleus GO:0005634 IDA
    cell surface GO:0009986 ISO
    extracellular space GO:0005615 ISO
    intracellular GO:0005622 ISO
    nucleoplasm GO:0005654 ISO
    extracellular region GO:0005576 ISO
 Experiment description of studies that identified Mif in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Mif
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mif is involved
No pathways found





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