Gene description for Rab13
Gene name RAB13, member RAS oncogene family
Gene symbol Rab13
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rab13
ExoCarta ExoCarta_81756
Vesiclepedia VP_81756
Entrez Gene 81756
UniProt P35286  
 Rab13 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Rab13
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    protein kinase A catalytic subunit binding GO:0034236 IPI
Biological Process
    exocytosis GO:0006887 IBA
    Golgi to plasma membrane transport GO:0006893 IEA
    Golgi to plasma membrane transport GO:0006893 ISO
    protein kinase A signaling GO:0010737 IBA
    protein kinase A signaling GO:0010737 IEA
    protein kinase A signaling GO:0010737 IMP
    protein kinase A signaling GO:0010737 ISO
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 ISO
    endosomal transport GO:0016197 ISS
    cortical actin cytoskeleton organization GO:0030866 IMP
    neuron projection development GO:0031175 IEA
    neuron projection development GO:0031175 IMP
    neuron projection development GO:0031175 ISO
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 IEA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    cellular response to insulin stimulus GO:0032869 IMP
    endothelial cell chemotaxis GO:0035767 IEA
    endothelial cell chemotaxis GO:0035767 ISO
    endothelial cell chemotaxis GO:0035767 ISS
    glucose homeostasis GO:0042593 IC
    trans-Golgi network to recycling endosome transport GO:0044795 ISO
    trans-Golgi network to recycling endosome transport GO:0044795 ISS
    positive regulation of D-glucose import GO:0046326 IC
    bicellular tight junction assembly GO:0070830 IEA
    bicellular tight junction assembly GO:0070830 ISO
    bicellular tight junction assembly GO:0070830 ISS
    protein localization to plasma membrane GO:0072659 IDA
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISS
    establishment of Sertoli cell barrier GO:0097368 IMP
    protein localization to cell leading edge GO:1902463 IEA
    protein localization to cell leading edge GO:1902463 ISO
    protein localization to cell leading edge GO:1902463 ISS
Subcellular Localization
    endosome GO:0005768 IBA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    trans-Golgi network GO:0005802 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell-cell junction GO:0005911 ISO
    bicellular tight junction GO:0005923 IEA
    bicellular tight junction GO:0005923 ISO
    bicellular tight junction GO:0005923 ISS
    synaptic vesicle GO:0008021 IBA
    lateral plasma membrane GO:0016328 IEA
    lateral plasma membrane GO:0016328 ISO
    lateral plasma membrane GO:0016328 ISS
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    endocytic vesicle GO:0030139 IEA
    endocytic vesicle GO:0030139 ISO
    endocytic vesicle GO:0030139 ISS
    trans-Golgi network transport vesicle GO:0030140 IBA
    cytoplasmic vesicle membrane GO:0030659 TAS
    cytoplasmic vesicle GO:0031410 ISO
    insulin-responsive compartment GO:0032593 IDA
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    neuron projection GO:0043005 ISS
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISS
    recycling endosome membrane GO:0055038 IEA
 Experiment description of studies that identified Rab13 in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 100
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rab13
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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