Gene description for Rnpep
Gene name arginyl aminopeptidase (aminopeptidase B)
Gene symbol Rnpep
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rnpep
ExoCarta ExoCarta_81761
Vesiclepedia VP_81761
Entrez Gene 81761
UniProt O09175  
 Rnpep identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rnpep
Molecular Function
    aminopeptidase activity GO:0004177 IDA
    copper ion binding GO:0005507 IDA
    metallopeptidase activity GO:0008237 IEA
    zinc ion binding GO:0008270 IDA
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 NAS
    peptide binding GO:0042277 IDA
    cobalt ion binding GO:0050897 IDA
    metalloaminopeptidase activity GO:0070006 IBA
    metalloaminopeptidase activity GO:0070006 IDA
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IC
    proteolysis GO:0006508 IEA
    protein processing GO:0016485 IDA
    protein processing GO:0016485 NAS
    negative regulation of blood pressure GO:0045776 IMP
    retina development in camera-type eye GO:0060041 IEP
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    Golgi apparatus GO:0005794 NAS
    plasma membrane GO:0005886 IDA
    external side of plasma membrane GO:0009897 IDA
    secretory granule GO:0030141 IDA
 Experiment description of studies that identified Rnpep in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rnpep
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rnpep is involved
No pathways found





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