Gene description for Rock1
Gene name Rho-associated coiled-coil containing protein kinase 1
Gene symbol Rock1
Other names/aliases P160Rock
ROCK-I
Species Rattus norvegicus
 Database cross references - Rock1
ExoCarta ExoCarta_81762
Vesiclepedia VP_81762
Entrez Gene 81762
UniProt Q63644  
 Rock1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rock1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    protein serine/threonine kinase activity GO:0004674 NAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    ATP binding GO:0005524 IEA
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 IMP
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IBA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 ISO
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    mitotic cytokinesis GO:0000281 IBA
    epithelial to mesenchymal transition GO:0001837 IEA
    epithelial to mesenchymal transition GO:0001837 ISO
    aortic valve morphogenesis GO:0003180 ISO
    apical constriction GO:0003383 IEA
    apical constriction GO:0003383 ISO
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    apoptotic process GO:0006915 IEA
    cytoskeleton organization GO:0007010 IMP
    leukocyte cell-cell adhesion GO:0007159 ISO
    Rho protein signal transduction GO:0007266 IBA
    Rho protein signal transduction GO:0007266 IEA
    Rho protein signal transduction GO:0007266 IMP
    Rho protein signal transduction GO:0007266 ISO
    positive regulation of autophagy GO:0010508 IEA
    positive regulation of autophagy GO:0010508 ISO
    positive regulation of cardiac muscle hypertrophy GO:0010613 IEA
    positive regulation of cardiac muscle hypertrophy GO:0010613 ISO
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of phosphatase activity GO:0010922 ISO
    negative regulation of angiogenesis GO:0016525 IEA
    negative regulation of angiogenesis GO:0016525 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
    membrane to membrane docking GO:0022614 IEA
    membrane to membrane docking GO:0022614 ISO
    actin cytoskeleton organization GO:0030036 IEA
    actin cytoskeleton organization GO:0030036 ISO
    actin cytoskeleton organization GO:0030036 ISO
    regulation of cell migration GO:0030334 IEA
    regulation of cell migration GO:0030334 ISO
    regulation of cell migration GO:0030334 ISS
    cortical actin cytoskeleton organization GO:0030866 IBA
    actomyosin structure organization GO:0031032 IBA
    neuron projection development GO:0031175 ISO
    bleb assembly GO:0032060 IEA
    bleb assembly GO:0032060 ISO
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of actin cytoskeleton organization GO:0032956 IEA
    regulation of actin cytoskeleton organization GO:0032956 ISO
    regulation of actin cytoskeleton organization GO:0032956 ISS
    regulation of actin filament-based process GO:0032970 ISO
    positive regulation of dephosphorylation GO:0035306 IEA
    positive regulation of dephosphorylation GO:0035306 ISO
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of MAPK cascade GO:0043410 ISO
    regulation of keratinocyte differentiation GO:0045616 IEA
    regulation of keratinocyte differentiation GO:0045616 ISO
    regulation of neuron differentiation GO:0045664 IEA
    regulation of neuron differentiation GO:0045664 ISO
    embryonic morphogenesis GO:0048598 IBA
    leukocyte migration GO:0050900 ISO
    leukocyte tethering or rolling GO:0050901 IEA
    leukocyte tethering or rolling GO:0050901 ISO
    myoblast migration GO:0051451 IEA
    myoblast migration GO:0051451 ISO
    myoblast migration GO:0051451 ISS
    positive regulation of focal adhesion assembly GO:0051894 IEA
    positive regulation of focal adhesion assembly GO:0051894 ISO
    positive regulation of focal adhesion assembly GO:0051894 ISS
    mRNA destabilization GO:0061157 IEA
    mRNA destabilization GO:0061157 ISO
    negative regulation of biomineral tissue development GO:0070168 ISO
    regulation of microtubule cytoskeleton organization GO:0070507 IEA
    regulation of microtubule cytoskeleton organization GO:0070507 ISO
    regulation of microtubule cytoskeleton organization GO:0070507 ISS
    response to transforming growth factor beta GO:0071559 IEA
    response to transforming growth factor beta GO:0071559 IGI
    response to transforming growth factor beta GO:0071559 ISO
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    protein localization to plasma membrane GO:0072659 IEA
    protein localization to plasma membrane GO:0072659 ISO
    regulation of synapse maturation GO:0090128 IEA
    regulation of synapse maturation GO:0090128 ISO
    podocyte cell migration GO:0090521 IEA
    podocyte cell migration GO:0090521 ISO
    podocyte cell migration GO:0090521 ISS
    motor neuron apoptotic process GO:0097049 IEA
    motor neuron apoptotic process GO:0097049 ISO
    blood vessel diameter maintenance GO:0097746 IEA
    blood vessel diameter maintenance GO:0097746 ISO
    regulation of angiotensin-activated signaling pathway GO:0110061 IEA
    regulation of angiotensin-activated signaling pathway GO:0110061 ISO
    neuron projection arborization GO:0140058 IEA
    neuron projection arborization GO:0140058 ISO
    positive regulation of protein localization to nucleus GO:1900182 ISO
    positive regulation of amyloid-beta clearance GO:1900223 IEA
    positive regulation of amyloid-beta clearance GO:1900223 ISO
    regulation of synaptic vesicle endocytosis GO:1900242 IEA
    regulation of synaptic vesicle endocytosis GO:1900242 ISO
    regulation of cell junction assembly GO:1901888 IBA
    negative regulation of amyloid-beta formation GO:1902430 IEA
    negative regulation of amyloid-beta formation GO:1902430 ISO
    negative regulation of amyloid precursor protein catabolic process GO:1902992 ISO
    regulation of establishment of endothelial barrier GO:1903140 IEA
    regulation of establishment of endothelial barrier GO:1903140 ISO
    negative regulation of bicellular tight junction assembly GO:1903347 IEA
    negative regulation of bicellular tight junction assembly GO:1903347 ISO
    positive regulation of connective tissue replacement GO:1905205 IEA
    positive regulation of connective tissue replacement GO:1905205 ISO
    response to angiotensin GO:1990776 IEA
    response to angiotensin GO:1990776 ISO
    negative regulation of motor neuron apoptotic process GO:2000672 IEA
    negative regulation of motor neuron apoptotic process GO:2000672 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    ruffle GO:0001726 IEA
    ruffle GO:0001726 ISO
    ruffle GO:0001726 ISS
    cytoplasm GO:0005737 IBA
    centriole GO:0005814 IEA
    cytoskeleton GO:0005856 IBA
    cytoskeleton GO:0005856 ISO
    cytoskeleton GO:0005856 ISS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cytoplasmic stress granule GO:0010494 IBA
    cytoplasmic stress granule GO:0010494 IEA
    cytoplasmic stress granule GO:0010494 ISO
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    lamellipodium GO:0030027 ISS
    bleb GO:0032059 IEA
    synapse GO:0045202 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    Schaffer collateral - CA1 synapse GO:0098685 ISO
 Experiment description of studies that identified Rock1 in sEVs
1
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rock1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Rock1 is involved
No pathways found





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