Gene description for Rock1
Gene name Rho-associated coiled-coil containing protein kinase 1
Gene symbol Rock1
Other names/aliases P160Rock
ROCK-I
Species Rattus norvegicus
 Database cross references - Rock1
ExoCarta ExoCarta_81762
Entrez Gene 81762
UniProt Q63644  
 Rock1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Rock1
Molecular Function
    ATP binding GO:0005524 IEA
    protein serine/threonine kinase activity GO:0004674 NAS
    metal ion binding GO:0046872 IEA
    GTP-Rho binding GO:0017049 IMP
Biological Process
    apoptotic process GO:0006915 IEA
    Rho protein signal transduction GO:0007266 IMP
    negative regulation of neuron apoptotic process GO:0043524 ISO
    myoblast migration GO:0051451 ISS
    leukocyte migration GO:0050900 ISO
    protein phosphorylation GO:0006468 IEA
    regulation of establishment of cell polarity GO:2000114 IEA
    regulation of stress fiber assembly GO:0051492 IEA
    membrane to membrane docking GO:0022614 ISO
    bleb assembly GO:0032060 ISO
    negative regulation of protein binding GO:0032091 ISO
    regulation of actin filament-based process GO:0032970 ISO
    regulation of keratinocyte differentiation GO:0045616 ISO
    cytoskeleton organization GO:0007010 IMP
    apical constriction GO:0003383 ISO
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of focal adhesion assembly GO:0051894 ISS
    negative regulation of angiogenesis GO:0016525 ISO
    leukocyte tethering or rolling GO:0050901 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
Subcellular Localization
    bleb GO:0032059 IEA
    centriole GO:0005814 IEA
    lamellipodium GO:0030027 ISS
    Golgi membrane GO:0000139 IEA
    cytoskeleton GO:0005856 ISS
    plasma membrane GO:0005886 ISS
    intracellular GO:0005622 IEA
    ruffle GO:0001726 ISS
 Experiment description of studies that identified Rock1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rock1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rock1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here