ExoCarta: Gene summary

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Gene description for Rpl36al
Gene name	ribosomal protein L36a-like
Gene symbol	Rpl36al
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Rpl36al
ExoCarta	ExoCarta_81769
Entrez Gene	81769
UniProt	P83883
Rpl36al identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Rpl36al

Molecular Function
	structural constituent of ribosome	GO:0003735	IEA
	molecular_function	GO:0003674	ND
Biological Process
	response to organic substance	GO:0010033	ISO
	biological_process	GO:0008150	ND
	translation	GO:0006412	IEA
	response to retinoic acid	GO:0032526	ISO
Subcellular Localization
	cytosolic large ribosomal subunit	GO:0022625	IBA

Experiment description of studies that identified Rpl36al in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting