Gene description for Slc20a1
Gene name solute carrier family 20 (phosphate transporter), member 1
Gene symbol Slc20a1
Other names/aliases PiT-1
Species Rattus norvegicus
 Database cross references - Slc20a1
ExoCarta ExoCarta_81826
Entrez Gene 81826
UniProt Q9JJP0  
 Slc20a1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Slc20a1
Molecular Function
    high-affinity inorganic phosphate:sodium symporter activity GO:0005316 IDA
    inorganic phosphate transmembrane transporter activity GO:0005315 IEA
    signal transducer activity GO:0004871 ISO
Biological Process
    phosphate ion transmembrane transport GO:0035435 IDA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    signal transduction GO:0007165 ISO
    phosphate ion transport GO:0006817 IDA
    sodium ion transmembrane transport GO:0035725 IDA
    sodium ion transport GO:0006814 IDA
Subcellular Localization
    plasma membrane GO:0005886 ISO
    integral component of membrane GO:0016021 IDA
 Experiment description of studies that identified Slc20a1 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Slc20a1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Slc20a1 is involved
PathwayEvidenceSource
Sodium-coupled phosphate cotransporters IEA Reactome





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