Gene description for SYMPK
Gene name symplekin
Gene symbol SYMPK
Other names/aliases SPK
SYM
Species Homo sapiens
 Database cross references - SYMPK
ExoCarta ExoCarta_8189
Vesiclepedia VP_8189
Entrez Gene 8189
HGNC 22935
MIM 602388
UniProt Q92797  
 SYMPK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for SYMPK
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mRNA processing GO:0006397 IEA
    cell adhesion GO:0007155 IEA
    negative regulation of protein binding GO:0032091 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    mRNA cleavage and polyadenylation specificity factor complex GO:0005847 IBA
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IDA
    bicellular tight junction GO:0005923 IEA
    nuclear body GO:0016604 IEA
    nuclear stress granule GO:0097165 IDA
 Experiment description of studies that identified SYMPK in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 274
MISEV standards
EM
Biophysical techniques
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
AIF
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
10
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SYMPK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPA 6626
Affinity Capture-MS Homo sapiens
2 LYPD3 27076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 S100A6 6277
Affinity Capture-MS Homo sapiens
4 KLC3  
Affinity Capture-MS Homo sapiens
5 P4HA2 8974
Affinity Capture-MS Homo sapiens
6 MKNK2 2872
Affinity Capture-MS Homo sapiens
7 IGHM 3507
Affinity Capture-MS Homo sapiens
8 DHX8 1659
Proximity Label-MS Homo sapiens
9 RPL35A 6165
Affinity Capture-MS Homo sapiens
10 TRIM55  
Two-hybrid Homo sapiens
11 DNAJB6 10049
Affinity Capture-MS Homo sapiens
12 KIAA1429 25962
Affinity Capture-MS Homo sapiens
13 VPS26A 9559
Affinity Capture-MS Homo sapiens
14 CPSF2 53981
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EFNA4  
Affinity Capture-MS Homo sapiens
16 RFXANK  
Affinity Capture-MS Homo sapiens
17 CPSF4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SEMA4C 54910
Affinity Capture-MS Homo sapiens
19 CXCR4 7852
Affinity Capture-MS Homo sapiens
20 USP33  
Affinity Capture-MS Homo sapiens
21 SLX4  
Affinity Capture-MS Homo sapiens
22 HYPM  
Affinity Capture-MS Homo sapiens
23 MECP2 4204
Affinity Capture-MS Homo sapiens
24 GLTSCR2  
Affinity Capture-MS Homo sapiens
25 SMARCAD1  
Affinity Capture-MS Homo sapiens
26 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MTA2 9219
Co-fractionation Homo sapiens
28 RBM14 10432
Affinity Capture-MS Homo sapiens
29 IFT20 90410
Affinity Capture-MS Homo sapiens
30 CPEB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 S100A2 6273
Affinity Capture-MS Homo sapiens
33 POLD1 5424
Affinity Capture-MS Homo sapiens
34 P4HA1 5033
Affinity Capture-MS Homo sapiens
35 MAPK3 5595
Affinity Capture-MS Homo sapiens
36 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
37 PLBD1 79887
Affinity Capture-MS Homo sapiens
38 FOXI2  
Affinity Capture-MS Homo sapiens
39 CSTF2 1478
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 UBE2I 7329
Biochemical Activity Homo sapiens
41 RPA2 6118
Affinity Capture-MS Homo sapiens
42 VCP 7415
Affinity Capture-MS Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 TRIM63  
Two-hybrid Homo sapiens
45 GPR182  
Affinity Capture-MS Homo sapiens
46 RAD18  
Affinity Capture-MS Homo sapiens
47 P4HB 5034
Affinity Capture-MS Homo sapiens
48 PML 5371
Affinity Capture-MS Homo sapiens
49 MBD3 53615
Co-fractionation Homo sapiens
50 CSTF3 1479
Affinity Capture-MS Homo sapiens
51 TMEM171  
Affinity Capture-MS Homo sapiens
52 FGFR1OP  
Affinity Capture-MS Homo sapiens
53 UBXN6 80700
Affinity Capture-MS Homo sapiens
54 PSG11  
Affinity Capture-MS Homo sapiens
55 FOXH1  
Affinity Capture-MS Homo sapiens
56 DNAAF2  
Affinity Capture-MS Homo sapiens
57 DCTN3 11258
Affinity Capture-MS Homo sapiens
58 CYB5B 80777
Affinity Capture-MS Homo sapiens
59 CPSF1 29894
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 ELMO3 79767
Affinity Capture-MS Homo sapiens
61 KHSRP 8570
Co-fractionation Homo sapiens
62 KMT2A  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
63 SIGLECL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 CSTF2T  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 C9orf78 51759
Affinity Capture-MS Homo sapiens
66 SMARCA5 8467
Co-fractionation Homo sapiens
67 SIRT7  
Affinity Capture-MS Homo sapiens
68 SNRPB 6628
Affinity Capture-MS Homo sapiens
69 NPTN 27020
Affinity Capture-MS Homo sapiens
70 WDR33 55339
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 FOXG1  
Affinity Capture-MS Homo sapiens
72 PPARD  
Affinity Capture-MS Homo sapiens
73 EGFR 1956
Negative Genetic Homo sapiens
74 USP24 23358
Co-fractionation Homo sapiens
75 FOXL1  
Affinity Capture-MS Homo sapiens
76 METTL21B  
Affinity Capture-MS Homo sapiens
77 FN1 2335
Affinity Capture-MS Homo sapiens
78 OCIAD1 54940
Affinity Capture-MS Homo sapiens
79 SAP30 8819
Co-fractionation Homo sapiens
80 C12orf74  
Affinity Capture-MS Homo sapiens
81 DNAI2  
Affinity Capture-MS Homo sapiens
82 FUS 2521
Affinity Capture-MS Homo sapiens
83 TOP1 7150
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
84 FIP1L1 81608
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 KAT6A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
86 FOXQ1  
Affinity Capture-MS Homo sapiens
87 GYPA  
Affinity Capture-MS Homo sapiens
88 MRPL42  
Affinity Capture-MS Homo sapiens
89 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
90 CLK1  
Affinity Capture-MS Homo sapiens
91 SIRT6  
Affinity Capture-MS Homo sapiens
92 CEP170P1  
Affinity Capture-MS Homo sapiens
93 CPSF6 11052
Affinity Capture-MS Homo sapiens
94 SSU72 29101
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
95 WWOX 51741
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
96 FOXB1  
Affinity Capture-MS Homo sapiens
97 KLHDC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 RPA1 6117
Affinity Capture-MS Homo sapiens
99 RP2 6102
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 PDHA1 5160
Affinity Capture-MS Homo sapiens
101 Id2  
Affinity Capture-MS Mus musculus
102 WIF1 11197
Affinity Capture-MS Homo sapiens
103 GSTM3 2947
Affinity Capture-MS Homo sapiens
104 KDM1A 23028
Co-fractionation Homo sapiens
105 HSF1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
106 CIT 11113
Affinity Capture-MS Homo sapiens
107 SERBP1 26135
Affinity Capture-MS Homo sapiens
108 SNW1 22938
Affinity Capture-MS Homo sapiens
109 KRAS 3845
Negative Genetic Homo sapiens
110 UBE3D  
Affinity Capture-MS Homo sapiens
111 NFKBIB  
Affinity Capture-MS Homo sapiens
112 SSRP1 6749
Affinity Capture-MS Homo sapiens
113 MEN1 4221
Affinity Capture-MS Homo sapiens
114 FOXE1  
Affinity Capture-MS Homo sapiens
115 RPA3 6119
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
116 CPSF3 51692
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
117 CDC5L 988
Affinity Capture-MS Homo sapiens
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