Gene description for Plekha2
Gene name pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 2
Gene symbol Plekha2
Other names/aliases 6430512N22Rik
AI851530
AU041791
TAPP2
W91773
Species Mus musculus
 Database cross references - Plekha2
ExoCarta ExoCarta_83436
Vesiclepedia VP_83436
Entrez Gene 83436
UniProt Q9ERS5  
 Plekha2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plekha2
Molecular Function
    fibronectin binding GO:0001968 IEA
    fibronectin binding GO:0001968 ISO
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IBA
    lipid binding GO:0008289 IEA
    PDZ domain binding GO:0030165 IPI
    laminin binding GO:0043236 IEA
    laminin binding GO:0043236 ISO
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IBA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 ISO
Biological Process
    positive regulation of cell-matrix adhesion GO:0001954 IEA
    positive regulation of cell-matrix adhesion GO:0001954 ISO
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    membrane GO:0016020 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Plekha2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plekha2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here