Gene description for Polr2f
Gene name polymerase (RNA) II (DNA directed) polypeptide F
Gene symbol Polr2f
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Polr2f
ExoCarta ExoCarta_83503
Entrez Gene 83503
UniProt O88828  
 Polr2f identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Polr2f
Molecular Function
    RNA polymerase I activity GO:0001054 IBA
    RNA polymerase II activity GO:0001055 IBA
    DNA-directed RNA polymerase activity GO:0003899 TAS
    DNA binding GO:0003677 IEA
    RNA polymerase III activity GO:0001056 IBA
    molecular_function GO:0003674 ND
Biological Process
    transcription from RNA polymerase II promoter GO:0006366 TAS
    biological_process GO:0008150 ND
    transcription from RNA polymerase I promoter GO:0006360 TAS
    transcription from RNA polymerase III promoter GO:0006383 TAS
Subcellular Localization
    nucleus GO:0005634 ISS
    cellular_component GO:0005575 ND
    DNA-directed RNA polymerase II, core complex GO:0005665 TAS
    DNA-directed RNA polymerase I complex GO:0005736 TAS
    DNA-directed RNA polymerase III complex GO:0005666 TAS
    nucleolus GO:0005730 ISO
 Experiment description of studies that identified Polr2f in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Polr2f
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Polr2f is involved
PathwayEvidenceSource
Cytosolic sensors of pathogen-associated DNA IEA Reactome
Dual incision reaction in TC-NER IEA Reactome
Formation of RNA Pol II elongation complex IEA Reactome
Formation of the Early Elongation Complex IEA Reactome
Formation of transcription-coupled NER (TC-NER) repair complex IEA Reactome
mRNA Capping IEA Reactome
mRNA Splicing - Major Pathway IEA Reactome
mRNA Splicing - Minor Pathway IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase I Chain Elongation IEA Reactome
RNA Polymerase I Promoter Escape IEA Reactome
RNA Polymerase I Transcription Initiation IEA Reactome
RNA Polymerase I Transcription Termination IEA Reactome
RNA Polymerase II Pre-transcription Events IEA Reactome
RNA Polymerase II Promoter Escape IEA Reactome
RNA Polymerase II Transcription Elongation IEA Reactome
RNA Polymerase II Transcription Initiation IEA Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance IEA Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening IEA Reactome
RNA Polymerase III Abortive And Retractive Initiation IEA Reactome
RNA Polymerase III Chain Elongation IEA Reactome
RNA Polymerase III Transcription Initiation From Type 1 Promoter IEA Reactome
RNA Polymerase III Transcription Initiation From Type 2 Promoter IEA Reactome
RNA Polymerase III Transcription Initiation From Type 3 Promoter IEA Reactome
RNA Polymerase III Transcription Termination IEA Reactome
Transcription-coupled NER (TC-NER) IEA Reactome
Transcriptional regulation by small RNAs IEA Reactome





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