Gene description for Polr2f
Gene name polymerase (RNA) II (DNA directed) polypeptide F
Gene symbol Polr2f
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Polr2f
ExoCarta ExoCarta_83503
Vesiclepedia VP_83503
Entrez Gene 83503
UniProt O88828  
 Polr2f identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Polr2f
Molecular Function
    RNA polymerase I activity GO:0001054 IBA
    RNA polymerase II activity GO:0001055 IBA
    RNA polymerase III activity GO:0001056 IBA
    DNA binding GO:0003677 IEA
    DNA-directed 5'-3' RNA polymerase activity GO:0003899 TAS
Biological Process
    transcription by RNA polymerase I GO:0006360 IBA
    transcription by RNA polymerase I GO:0006360 TAS
    transcription by RNA polymerase II GO:0006366 IBA
    transcription by RNA polymerase II GO:0006366 ISO
    transcription by RNA polymerase II GO:0006366 ISS
    transcription by RNA polymerase II GO:0006366 TAS
    transcription by RNA polymerase III GO:0006383 TAS
    biological_process GO:0008150 ND
    tRNA transcription by RNA polymerase III GO:0042797 IBA
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    RNA polymerase II, core complex GO:0005665 IBA
    RNA polymerase II, core complex GO:0005665 ISO
    RNA polymerase II, core complex GO:0005665 ISS
    RNA polymerase II, core complex GO:0005665 TAS
    RNA polymerase III complex GO:0005666 IBA
    RNA polymerase III complex GO:0005666 ISO
    RNA polymerase III complex GO:0005666 TAS
    RNA polymerase I complex GO:0005736 IBA
    RNA polymerase I complex GO:0005736 ISO
    RNA polymerase I complex GO:0005736 TAS
 Experiment description of studies that identified Polr2f in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Polr2f
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Polr2f is involved
PathwayEvidenceSource
B-WICH complex positively regulates rRNA expression IEA Reactome
DNA Repair IEA Reactome
Dual incision in TC-NER IEA Reactome
Epigenetic regulation of gene expression IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent gene expression IEA Reactome
FGFR2 alternative splicing IEA Reactome
Formation of RNA Pol II elongation complex IEA Reactome
Formation of TC-NER Pre-Incision Complex IEA Reactome
Formation of the Early Elongation Complex IEA Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER IEA Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA IEA Reactome
Generic Transcription Pathway IEA Reactome
Metabolism of RNA IEA Reactome
mRNA Capping IEA Reactome
mRNA Splicing IEA Reactome
mRNA Splicing - Major Pathway IEA Reactome
mRNA Splicing - Minor Pathway IEA Reactome
Nucleotide Excision Repair IEA Reactome
Positive epigenetic regulation of rRNA expression IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase I Promoter Clearance IEA Reactome
RNA Polymerase I Promoter Escape IEA Reactome
RNA Polymerase I Transcription IEA Reactome
RNA Polymerase I Transcription Initiation IEA Reactome
RNA Polymerase I Transcription Termination IEA Reactome
RNA Polymerase II Pre-transcription Events IEA Reactome
RNA Polymerase II Promoter Escape IEA Reactome
RNA polymerase II transcribes snRNA genes IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RNA Polymerase II Transcription Elongation IEA Reactome
RNA Polymerase II Transcription Initiation IEA Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance IEA Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening IEA Reactome
RNA Polymerase III Transcription IEA Reactome
RNA Polymerase III Transcription Initiation IEA Reactome
RNA Polymerase III Transcription Initiation From Type 1 Promoter IEA Reactome
RNA Polymerase III Transcription Initiation From Type 2 Promoter IEA Reactome
RNA Polymerase III Transcription Initiation From Type 3 Promoter IEA Reactome
Signal Transduction IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
TP53 Regulates Transcription of DNA Repair Genes IEA Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) IEA Reactome
Transcriptional regulation by small RNAs IEA Reactome
Transcriptional Regulation by TP53 IEA Reactome





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