Gene description for Tpp1
Gene name tripeptidyl peptidase I
Gene symbol Tpp1
Other names/aliases Cln2
Species Rattus norvegicus
 Database cross references - Tpp1
ExoCarta ExoCarta_83534
Vesiclepedia VP_83534
Entrez Gene 83534
UniProt Q9EQV6  
 Tpp1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tpp1
Molecular Function
    endopeptidase activity GO:0004175 IBA
    endopeptidase activity GO:0004175 IDA
    endopeptidase activity GO:0004175 ISO
    endopeptidase activity GO:0004175 ISS
    serine-type endopeptidase activity GO:0004252 IEA
    peptidase activity GO:0008233 ISO
    peptidase activity GO:0008233 ISS
    serine-type peptidase activity GO:0008236 ISO
    serine-type peptidase activity GO:0008236 ISS
    tripeptidyl-peptidase activity GO:0008240 IBA
    tripeptidyl-peptidase activity GO:0008240 IDA
    tripeptidyl-peptidase activity GO:0008240 IEA
    tripeptidyl-peptidase activity GO:0008240 ISO
    tripeptidyl-peptidase activity GO:0008240 ISS
    lysophosphatidic acid binding GO:0035727 IEA
    lysophosphatidic acid binding GO:0035727 ISO
    lysophosphatidic acid binding GO:0035727 ISS
    peptide binding GO:0042277 IDA
    metal ion binding GO:0046872 IEA
    sulfatide binding GO:0120146 IEA
    sulfatide binding GO:0120146 ISO
    sulfatide binding GO:0120146 ISS
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IEA
    proteolysis GO:0006508 ISO
    proteolysis GO:0006508 ISS
    lysosome organization GO:0007040 IEA
    lysosome organization GO:0007040 ISO
    nervous system development GO:0007399 IEA
    nervous system development GO:0007399 ISO
    nervous system development GO:0007399 ISS
    central nervous system development GO:0007417 IBA
    protein catabolic process GO:0030163 IDA
    epithelial cell differentiation GO:0030855 IEA
    epithelial cell differentiation GO:0030855 ISO
    peptide catabolic process GO:0043171 IEA
    peptide catabolic process GO:0043171 ISO
    peptide catabolic process GO:0043171 ISS
    bone resorption GO:0045453 IEA
    bone resorption GO:0045453 ISO
    bone resorption GO:0045453 ISS
    neuromuscular process controlling balance GO:0050885 IEA
    neuromuscular process controlling balance GO:0050885 ISO
    protein localization to chromosome, telomeric region GO:0070198 IEA
    protein localization to chromosome, telomeric region GO:0070198 ISO
    lysosomal protein catabolic process GO:1905146 IEA
    lysosomal protein catabolic process GO:1905146 ISO
Subcellular Localization
    lysosome GO:0005764 IEA
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    melanosome GO:0042470 IEA
    membrane raft GO:0045121 IEA
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISS
    recycling endosome GO:0055037 IEA
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISS
 Experiment description of studies that identified Tpp1 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tpp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tpp1 is involved
No pathways found





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