Gene description for Tpp1
Gene name tripeptidyl peptidase I
Gene symbol Tpp1
Other names/aliases Cln2
Species Rattus norvegicus
 Database cross references - Tpp1
ExoCarta ExoCarta_83534
Entrez Gene 83534
UniProt Q9EQV6  
 Tpp1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tpp1
Molecular Function
    peptide binding GO:0042277 IDA
    serine-type peptidase activity GO:0008236 ISS
    tripeptidyl-peptidase activity GO:0008240 ISS
    serine-type endopeptidase activity GO:0004252 IEA
    endopeptidase activity GO:0004175 ISS
    metal ion binding GO:0046872 IEA
    peptidase activity GO:0008233 ISS
Biological Process
    neuromuscular process controlling balance GO:0050885 ISO
    epithelial cell differentiation GO:0030855 ISO
    peptide catabolic process GO:0043171 ISS
    lysosome organization GO:0007040 ISO
    nervous system development GO:0007399 ISS
    proteolysis GO:0006508 ISS
    central nervous system development GO:0007417 IBA
    bone resorption GO:0045453 ISS
Subcellular Localization
    mitochondrion GO:0005739 ISO
    melanosome GO:0042470 IEA
    lysosome GO:0005764 ISS
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Tpp1 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tpp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tpp1 is involved
No pathways found





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