Gene description for Pafah1b1
Gene name platelet-activating factor acetylhydrolase 1b, regulatory subunit 1
Gene symbol Pafah1b1
Other names/aliases LIS1
Species Rattus norvegicus
 Database cross references - Pafah1b1
ExoCarta ExoCarta_83572
Vesiclepedia VP_83572
Entrez Gene 83572
UniProt P63004  
 Pafah1b1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pafah1b1
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 ISO
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 IPI
    dynein intermediate chain binding GO:0045505 IDA
    protein heterodimerization activity GO:0046982 ISS
    platelet-activating factor acetyltransferase activity GO:0047179 TAS
    microtubule plus-end binding GO:0051010 IBA
    phosphoprotein binding GO:0051219 ISO
    dynein complex binding GO:0070840 IBA
    dynein complex binding GO:0070840 ISO
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    establishment of mitotic spindle orientation GO:0000132 ISO
    microtubule cytoskeleton organization GO:0000226 ISO
    ameboidal-type cell migration GO:0001667 ISO
    acrosome assembly GO:0001675 ISO
    neuron migration GO:0001764 IMP
    neuron migration GO:0001764 ISO
    neuron migration GO:0001764 ISO
    positive regulation of cytokine-mediated signaling pathway GO:0001961 ISO
    microtubule-based process GO:0007017 ISO
    nuclear migration GO:0007097 IBA
    nuclear migration GO:0007097 IMP
    JNK cascade GO:0007254 ISO
    chemical synaptic transmission GO:0007268 ISO
    germ cell development GO:0007281 IBA
    neuroblast proliferation GO:0007405 ISO
    learning or memory GO:0007611 ISO
    retrograde axonal transport GO:0008090 IBA
    retrograde axonal transport GO:0008090 ISO
    adult locomotory behavior GO:0008344 ISO
    adult locomotory behavior GO:0008344 ISO
    protein secretion GO:0009306 ISO
    negative regulation of neuron projection development GO:0010977 IMP
    lipid catabolic process GO:0016042 IEA
    cell migration GO:0016477 ISO
    stem cell division GO:0017145 IMP
    transmission of nerve impulse GO:0019226 ISO
    corpus callosum morphogenesis GO:0021540 ISO
    hippocampus development GO:0021766 ISO
    layer formation in cerebral cortex GO:0021819 ISO
    cerebral cortex neuron differentiation GO:0021895 IMP
    cerebral cortex development GO:0021987 ISO
    cerebral cortex development GO:0021987 ISO
    actin cytoskeleton organization GO:0030036 ISO
    microtubule organizing center organization GO:0031023 IBA
    microtubule organizing center organization GO:0031023 ISO
    osteoclast development GO:0036035 ISO
    reelin-mediated signaling pathway GO:0038026 ISO
    reelin-mediated signaling pathway GO:0038026 ISS
    positive regulation of embryonic development GO:0040019 ISO
    establishment of planar polarity of embryonic epithelium GO:0042249 ISO
    cortical microtubule organization GO:0043622 ISO
    positive regulation of axon extension GO:0045773 IMP
    positive regulation of mitotic cell cycle GO:0045931 IMP
    negative regulation of JNK cascade GO:0046329 ISO
    vesicle transport along microtubule GO:0047496 IBA
    vesicle transport along microtubule GO:0047496 ISO
    brain morphogenesis GO:0048854 IBA
    brain morphogenesis GO:0048854 ISO
    modulation of chemical synaptic transmission GO:0050804 ISO
    neuromuscular process controlling balance GO:0050885 ISO
    neuromuscular process controlling balance GO:0050885 ISO
    microtubule sliding GO:0051012 IEA
    nuclear membrane disassembly GO:0051081 ISO
    positive regulation of cellular component organization GO:0051130 ISO
    establishment of localization in cell GO:0051649 ISO
    establishment of centrosome localization GO:0051660 IMP
    maintenance of centrosome location GO:0051661 ISO
    auditory receptor cell development GO:0060117 ISO
    positive regulation of dendritic spine morphogenesis GO:0061003 ISO
    regulation of microtubule cytoskeleton organization GO:0070507 ISO
    cochlea development GO:0090102 ISO
    microtubule cytoskeleton organization involved in establishment of planar polarity GO:0090176 ISO
    myeloid leukocyte migration GO:0097529 ISO
    regulation of postsynapse organization GO:0099175 ISO
    radial glia-guided pyramidal neuron migration GO:0140650 ISO
    interneuron migration GO:1904936 ISO
Subcellular Localization
    astral microtubule GO:0000235 ISO
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 ISO
    nuclear envelope GO:0005635 IBA
    nuclear envelope GO:0005635 IDA
    nuclear envelope GO:0005635 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    kinesin complex GO:0005871 IDA
    microtubule associated complex GO:0005875 IBA
    microtubule associated complex GO:0005875 ISO
    cytoplasmic microtubule GO:0005881 IBA
    cell cortex GO:0005938 ISO
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 IDA
    1-alkyl-2-acetylglycerophosphocholine esterase complex GO:0008247 ISS
    microtubule cytoskeleton GO:0015630 ISO
    axon GO:0030424 IDA
    growth cone GO:0030426 IDA
    cell leading edge GO:0031252 ISO
    motile cilium GO:0031514 ISO
    nuclear membrane GO:0031965 IEA
    vesicle GO:0031982 IDA
    stereocilium GO:0032420 ISO
    neuron projection GO:0043005 IBA
    neuronal cell body GO:0043025 IBA
    neuronal cell body GO:0043025 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    central region of growth cone GO:0090724 IDA
    non-motile cilium GO:0097730 ISO
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    glutamatergic synapse GO:0098978 ISO
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified Pafah1b1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pafah1b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pafah1b1 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
G2/M Transition IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Vesicle-mediated transport IEA Reactome





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