Gene description for Pafah1b1
Gene name platelet-activating factor acetylhydrolase 1b, regulatory subunit 1
Gene symbol Pafah1b1
Other names/aliases LIS1
Species Rattus norvegicus
 Database cross references - Pafah1b1
ExoCarta ExoCarta_83572
Entrez Gene 83572
UniProt P63004  
 Pafah1b1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Pafah1b1
Molecular Function
    dynein intermediate chain binding GO:0045505 IDA
    protein homodimerization activity GO:0042803 ISO
    microtubule binding GO:0008017 ISO
    protein complex binding GO:0032403 IPI
    protein binding GO:0005515 IPI
    dynein binding GO:0045502 ISO
    platelet-activating factor acetyltransferase activity GO:0047179 TAS
    phosphoprotein binding GO:0051219 ISO
Biological Process
    nuclear migration GO:0007097 IMP
    transmission of nerve impulse GO:0019226 ISO
    regulation of GTPase activity GO:0043087 ISO
    neuromuscular process controlling balance GO:0050885 ISO
    positive regulation of mitotic cell cycle GO:0045931 IMP
    acrosome assembly GO:0001675 ISO
    vesicle transport along microtubule GO:0047496 ISO
    brain development GO:0007420 IEP
    microtubule organizing center organization GO:0031023 ISO
    synaptic transmission GO:0007268 ISO
    actin cytoskeleton organization GO:0030036 ISO
    learning or memory GO:0007611 ISO
    lipid catabolic process GO:0016042 IEA
    hippocampus development GO:0021766 ISO
    neuroblast proliferation GO:0007405 ISO
    brain morphogenesis GO:0048854 ISO
    establishment of centrosome localization GO:0051660 IMP
    cell migration GO:0016477 ISO
    nuclear envelope disassembly GO:0051081 ISO
    cerebral cortex development GO:0021987 ISO
    retrograde axon cargo transport GO:0008090 ISO
    positive regulation of cytokine-mediated signaling pathway GO:0001961 ISO
    corpus callosum morphogenesis GO:0021540 ISO
    microtubule-based process GO:0007017 ISO
    negative regulation of neuron projection development GO:0010977 IMP
    establishment of mitotic spindle orientation GO:0000132 ISO
    osteoclast development GO:0036035 ISO
    stem cell division GO:0017145 IMP
    positive regulation of axon extension GO:0045773 IMP
    negative regulation of JNK cascade GO:0046329 ISO
    cerebral cortex neuron differentiation GO:0021895 IMP
    layer formation in cerebral cortex GO:0021819 ISO
    protein secretion GO:0009306 ISO
    neuron migration GO:0001764 ISO
    mitotic nuclear division GO:0007067 IEA
    ameboidal-type cell migration GO:0001667 ISO
    adult locomotory behavior GO:0008344 ISO
    microtubule cytoskeleton organization GO:0000226 ISO
Subcellular Localization
    kinetochore GO:0000776 ISO
    microtubule cytoskeleton GO:0015630 ISO
    vesicle GO:0031982 IDA
    cytoplasm GO:0005737 ISO
    nonmotile primary cilium GO:0031513 ISO
    cytosol GO:0005829 IDA
    nuclear membrane GO:0031965 IEA
    neuron projection GO:0043005 IDA
    motile primary cilium GO:0031512 ISO
    cell GO:0005623 ISO
    nuclear envelope GO:0005635 ISO
    neuronal cell body GO:0043025 IDA
    cell leading edge GO:0031252 ISO
    microtubule associated complex GO:0005875 ISO
    intracellular GO:0005622 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    centrosome GO:0005813 ISO
    axon GO:0030424 IDA
    kinesin complex GO:0005871 IDA
    extracellular exosome GO:0070062 ISO
    astral microtubule GO:0000235 ISO
    growth cone GO:0030426 IDA
    cell cortex GO:0005938 ISO
 Experiment description of studies that identified Pafah1b1 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Pafah1b1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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