Gene description for Clcn3
Gene name chloride channel, voltage-sensitive 3
Gene symbol Clcn3
Other names/aliases ClC-3
Species Rattus norvegicus
 Database cross references - Clcn3
ExoCarta ExoCarta_84360
Entrez Gene 84360
UniProt P51792  
 Clcn3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Clcn3
Molecular Function
    chloride channel activity GO:0005254 ISO
    antiporter activity GO:0015297 IEA
    ion channel activity GO:0005216 IDA
    PDZ domain binding GO:0030165 ISS
    volume-sensitive chloride channel activity GO:0072320 ISO
    voltage-gated ion channel activity GO:0005244 IDA
    ATP binding GO:0005524 IEA
    protein heterodimerization activity GO:0046982 ISO
    voltage-gated chloride channel activity GO:0005247 IDA
Biological Process
    regulation of ion transmembrane transport GO:0034765 IDA
    photoreceptor cell maintenance GO:0045494 ISO
    regulation of anion transport GO:0044070 ISO
    adult locomotory behavior GO:0008344 ISO
    chloride transport GO:0006821 IDA
    chloride transmembrane transport GO:1902476 ISO
    synaptic transmission, GABAergic GO:0051932 ISO
    regulation of anion transmembrane transport GO:1903959 IEA
    ion transport GO:0006811 IDA
    synaptic vesicle lumen acidification GO:0097401 ISO
    neuron cellular homeostasis GO:0070050 ISO
    negative regulation of cell volume GO:0045794 ISO
    phagocytosis, engulfment GO:0006911 ISO
    positive regulation of reactive oxygen species biosynthetic process GO:1903428 ISO
    synaptic transmission, glutamatergic GO:0035249 ISO
Subcellular Localization
    axon terminus GO:0043679 ISO
    cell GO:0005623 ISO
    early endosome membrane GO:0031901 IEA
    vesicle membrane GO:0012506 ISS
    plasma membrane GO:0005886 ISO
    late endosome GO:0005770 ISS
    secretory granule GO:0030141 ISO
    cytoplasmic vesicle GO:0031410 ISO
    transport vesicle membrane GO:0030658 IEA
    apical plasma membrane GO:0016324 IDA
    synaptic vesicle lumen GO:0034592 ISO
    integral component of membrane GO:0016021 IDA
    late endosome membrane GO:0031902 IEA
    phagocytic vesicle GO:0045335 ISO
    intracellular GO:0005622 ISO
    early endosome GO:0005769 ISS
    synaptic vesicle GO:0008021 ISO
    external side of plasma membrane GO:0009897 ISO
    Golgi apparatus GO:0005794 ISS
    membrane GO:0016020 ISO
    synapse GO:0045202 IDA
    inhibitory synapse GO:0060077 IDA
    endosome GO:0005768 ISO
    specific granule GO:0042581 ISO
 Experiment description of studies that identified Clcn3 in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Clcn3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Clcn3 is involved
PathwayEvidenceSource
Stimuli-sensing channels IEA Reactome





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