Gene description for Gnpat
Gene name glyceronephosphate O-acyltransferase
Gene symbol Gnpat
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gnpat
ExoCarta ExoCarta_84470
Entrez Gene 84470
UniProt Q9ES71  
 Gnpat identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gnpat
Molecular Function
    catalytic activity GO:0003824 ISO
    receptor binding GO:0005102 ISO
    glycerone-phosphate O-acyltransferase activity GO:0016287 TAS
    palmitoyl-CoA hydrolase activity GO:0016290 ISO
Biological Process
    cerebellum morphogenesis GO:0021587 ISO
    ether lipid biosynthetic process GO:0008611 ISO
    response to nutrient GO:0007584 IEP
    paranodal junction assembly GO:0030913 ISO
    response to drug GO:0042493 IEP
    synapse assembly GO:0007416 ISO
    response to fatty acid GO:0070542 IEP
    myelination GO:0042552 ISO
    response to starvation GO:0042594 IEP
    glycerophospholipid metabolic process GO:0006650 IEA
    membrane organization GO:0061024 ISO
Subcellular Localization
    peroxisome GO:0005777 ISO
    peroxisomal membrane GO:0005778 TAS
    mitochondrion GO:0005739 IDA
    membrane GO:0016020 ISO
 Experiment description of studies that identified Gnpat in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gnpat
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gnpat is involved
PathwayEvidenceSource
Plasmalogen biosynthesis IEA Reactome
Synthesis of PA IEA Reactome





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