Gene description for Gnpat
Gene name glyceronephosphate O-acyltransferase
Gene symbol Gnpat
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gnpat
ExoCarta ExoCarta_84470
Vesiclepedia VP_84470
Entrez Gene 84470
UniProt Q9ES71  
 Gnpat identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gnpat
Molecular Function
    catalytic activity GO:0003824 ISO
    glycerone-phosphate O-acyltransferase activity GO:0016287 IBA
    glycerone-phosphate O-acyltransferase activity GO:0016287 IEA
    glycerone-phosphate O-acyltransferase activity GO:0016287 ISO
    glycerone-phosphate O-acyltransferase activity GO:0016287 TAS
Biological Process
    glycerophospholipid metabolic process GO:0006650 IEA
    synapse assembly GO:0007416 ISO
    response to nutrient GO:0007584 IEP
    ether lipid biosynthetic process GO:0008611 IBA
    ether lipid biosynthetic process GO:0008611 IEA
    ether lipid biosynthetic process GO:0008611 ISO
    ether lipid biosynthetic process GO:0008611 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    cerebellum morphogenesis GO:0021587 ISO
    paranodal junction assembly GO:0030913 ISO
    myelination GO:0042552 ISO
    response to starvation GO:0042594 IEP
    membrane organization GO:0061024 ISO
    response to fatty acid GO:0070542 IEP
Subcellular Localization
    peroxisome GO:0005777 IDA
    peroxisome GO:0005777 ISO
    peroxisomal membrane GO:0005778 IBA
    peroxisomal membrane GO:0005778 IDA
    peroxisomal membrane GO:0005778 IEA
    peroxisomal membrane GO:0005778 TAS
 Experiment description of studies that identified Gnpat in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gnpat
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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