Gene description for Fen1
Gene name flap structure-specific endonuclease 1
Gene symbol Fen1
Other names/aliases FEN-1
Species Rattus norvegicus
 Database cross references - Fen1
ExoCarta ExoCarta_84490
Entrez Gene 84490
UniProt Q5XIP6  
 Fen1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Fen1
Molecular Function
    magnesium ion binding GO:0000287 ISO
    manganese ion binding GO:0030145 ISO
    5'-3' exonuclease activity GO:0008409 ISO
    RNA-DNA hybrid ribonuclease activity GO:0004523 ISO
    flap endonuclease activity GO:0048256 ISO
    exonuclease activity GO:0004527 NAS
    5'-flap endonuclease activity GO:0017108 ISO
    DNA binding GO:0003677 ISO
Biological Process
    nucleic acid phosphodiester bond hydrolysis GO:0090305 ISO
    memory GO:0007613 IMP
    RNA phosphodiester bond hydrolysis, endonucleolytic GO:0090502 ISO
    DNA repair GO:0006281 ISO
    DNA replication GO:0006260 ISO
    base-excision repair GO:0006284 IEA
    DNA replication, removal of RNA primer GO:0043137 ISO
    DNA metabolic process GO:0006259 ISO
Subcellular Localization
    protein complex GO:0043234 ISO
    plasma membrane GO:0005886 ISO
    nucleus GO:0005634 ISO
    mitochondrion GO:0005739 ISO
    nucleolus GO:0005730 ISO
    nucleoplasm GO:0005654 IEA
    membrane GO:0016020 ISO
 Experiment description of studies that identified Fen1 in exosomes
1
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Fen1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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