Gene description for Dgat1
Gene name diacylglycerol O-acyltransferase 1
Gene symbol Dgat1
Other names/aliases ARAT
Dgat
Species Rattus norvegicus
 Database cross references - Dgat1
ExoCarta ExoCarta_84497
Vesiclepedia VP_84497
Entrez Gene 84497
UniProt Q9ERM3  
 Dgat1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Dgat1
Molecular Function
    2-acylglycerol O-acyltransferase activity GO:0003846 IEA
    2-acylglycerol O-acyltransferase activity GO:0003846 ISO
    diacylglycerol O-acyltransferase activity GO:0004144 IBA
    diacylglycerol O-acyltransferase activity GO:0004144 IDA
    diacylglycerol O-acyltransferase activity GO:0004144 IEA
    diacylglycerol O-acyltransferase activity GO:0004144 ISO
    fatty acid binding GO:0005504 IDA
    acyltransferase activity GO:0016746 IMP
    diacylglycerol binding GO:0019992 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    retinol O-fatty-acyltransferase activity GO:0050252 IEA
Biological Process
    lipid metabolic process GO:0006629 IEA
    monoacylglycerol biosynthetic process GO:0006640 IEA
    monoacylglycerol biosynthetic process GO:0006640 ISO
    monoacylglycerol biosynthetic process GO:0006640 ISS
    positive regulation of triglyceride biosynthetic process GO:0010867 IMP
    triglyceride biosynthetic process GO:0019432 IBA
    triglyceride biosynthetic process GO:0019432 IDA
    triglyceride biosynthetic process GO:0019432 IEA
    triglyceride biosynthetic process GO:0019432 ISO
    triglyceride biosynthetic process GO:0019432 ISO
    lipid storage GO:0019915 IEA
    lipid storage GO:0019915 ISO
    insulin secretion GO:0030073 IMP
    very-low-density lipoprotein particle assembly GO:0034379 IEA
    very-low-density lipoprotein particle assembly GO:0034379 ISO
    long-chain fatty-acyl-CoA metabolic process GO:0035336 IEA
    long-chain fatty-acyl-CoA metabolic process GO:0035336 ISO
    retinol metabolic process GO:0042572 IEA
    response to human chorionic gonadotropin GO:0044752 IEP
    positive regulation of fatty acid oxidation GO:0046321 IMP
    diacylglycerol metabolic process GO:0046339 IEA
    diacylglycerol metabolic process GO:0046339 ISO
    diacylglycerol metabolic process GO:0046339 ISS
    glycerolipid metabolic process GO:0046486 IDA
    fatty acid homeostasis GO:0055089 IEA
    fatty acid homeostasis GO:0055089 ISO
    regulation of vitamin A metabolic process GO:1901738 IMP
    ketone body metabolic process GO:1902224 IMP
    regulation of eating behavior GO:1903998 IMP
    regulation of intestinal lipid absorption GO:1904729 IMP
    positive regulation of hepatic stellate cell activation GO:2000491 IMP
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
    endoplasmic reticulum membrane GO:0005789 ISO
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified Dgat1 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Dgat1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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