Gene description for Ran
Gene name RAN, member RAS oncogene family
Gene symbol Ran
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ran
ExoCarta ExoCarta_84509
Vesiclepedia VP_84509
Entrez Gene 84509
UniProt P62828  
 Ran identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ran
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 ISO
    nuclear export signal receptor activity GO:0005049 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IDA
    GDP binding GO:0019003 ISO
    protein domain specific binding GO:0019904 IPI
    protein-containing complex binding GO:0044877 IPI
    dynein intermediate chain binding GO:0045505 IDA
    protein heterodimerization activity GO:0046982 ISO
    importin-alpha family protein binding GO:0061676 IDA
    pre-miRNA binding GO:0070883 ISO
    RISC complex binding GO:1905172 ISO
Biological Process
    ribosomal subunit export from nucleus GO:0000054 IBA
    ribosomal large subunit export from nucleus GO:0000055 ISO
    ribosomal small subunit export from nucleus GO:0000056 ISO
    mitotic sister chromatid segregation GO:0000070 ISO
    mitotic sister chromatid segregation GO:0000070 ISS
    protein import into nucleus GO:0006606 IBA
    protein import into nucleus GO:0006606 ISO
    protein import into nucleus GO:0006606 ISO
    protein import into nucleus GO:0006606 ISS
    protein import into nucleus GO:0006606 TAS
    protein export from nucleus GO:0006611 ISO
    protein export from nucleus GO:0006611 ISS
    protein export from nucleus GO:0006611 TAS
    nucleocytoplasmic transport GO:0006913 TAS
    spermatid development GO:0007286 IEP
    protein localization GO:0008104 ISO
    response to organic cyclic compound GO:0014070 IEP
    hippocampus development GO:0021766 IEP
    actin cytoskeleton organization GO:0030036 ISO
    protein-containing complex localization GO:0031503 ISO
    positive regulation of protein import into nucleus GO:0042307 ISO
    GTP metabolic process GO:0046039 ISO
    GTP metabolic process GO:0046039 ISS
    positive regulation of protein export from nucleus GO:0046827 ISO
    cell division GO:0051301 IEA
    snRNA import into nucleus GO:0061015 ISO
    snRNA import into nucleus GO:0061015 ISS
    cellular response to mineralocorticoid stimulus GO:0071389 IEP
    protein localization to nucleolus GO:1902570 ISO
Subcellular Localization
    chromatin GO:0000785 ISO
    male germ cell nucleus GO:0001673 IDA
    manchette GO:0002177 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nuclear envelope GO:0005635 ISO
    nuclear pore GO:0005643 IDA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    centriole GO:0005814 ISO
    cytosol GO:0005829 IEA
    RISC complex GO:0016442 ISO
    midbody GO:0030496 ISO
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 ISO
    sperm flagellum GO:0036126 IDA
    melanosome GO:0042470 IEA
    RNA nuclear export complex GO:0042565 ISO
    recycling endosome GO:0055037 ISO
    Flemming body GO:0090543 ISO
 Experiment description of studies that identified Ran in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 100
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 101
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ran
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Nutf2  
Reconstituted Complex Rattus norvegicus
2 Gja1  
Affinity Capture-MS Rattus norvegicus
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