Gene description for Xpo1
Gene name exportin 1
Gene symbol Xpo1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Xpo1
ExoCarta ExoCarta_85252
Vesiclepedia VP_85252
Entrez Gene 85252
UniProt Q80U96  
 Xpo1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Xpo1
Molecular Function
    RNA binding GO:0003723 IEA
    nuclear export signal receptor activity GO:0005049 IBA
    nuclear export signal receptor activity GO:0005049 IEA
    nuclear export signal receptor activity GO:0005049 ISO
    protein binding GO:0005515 IPI
    protein domain specific binding GO:0019904 IPI
    small GTPase binding GO:0031267 IEA
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    ribosomal subunit export from nucleus GO:0000054 ISO
    ribosomal large subunit export from nucleus GO:0000055 IBA
    ribosomal large subunit export from nucleus GO:0000055 IEA
    ribosomal large subunit export from nucleus GO:0000055 ISO
    ribosomal small subunit export from nucleus GO:0000056 IBA
    ribosomal small subunit export from nucleus GO:0000056 IEA
    ribosomal small subunit export from nucleus GO:0000056 ISO
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    mRNA export from nucleus GO:0006406 IEA
    mRNA export from nucleus GO:0006406 ISO
    protein export from nucleus GO:0006611 IDA
    protein export from nucleus GO:0006611 IEA
    protein export from nucleus GO:0006611 ISO
    protein export from nucleus GO:0006611 ISO
    nucleocytoplasmic transport GO:0006913 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    regulation of centrosome duplication GO:0010824 IEA
    regulation of centrosome duplication GO:0010824 ISO
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IEA
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 ISO
    protein localization to nucleus GO:0034504 IEA
    protein localization to nucleus GO:0034504 ISO
    regulation of protein catabolic process GO:0042176 ISO
    ribosome biogenesis GO:0042254 ISO
    regulation of protein export from nucleus GO:0046825 IEA
    regulation of protein export from nucleus GO:0046825 ISO
    cellular response to triglyceride GO:0071401 IEP
    cellular response to salt GO:1902075 IEP
Subcellular Localization
    kinetochore GO:0000776 IEA
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    annulate lamellae GO:0005642 IEA
    annulate lamellae GO:0005642 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Cajal body GO:0015030 IEA
    nuclear membrane GO:0031965 IEA
    nuclear membrane GO:0031965 ISO
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    ribonucleoprotein complex GO:1990904 IEA
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Xpo1 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Xpo1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Xpo1 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cyclin A/B1/B2 associated events during G2/M transition IEA Reactome
Deactivation of the beta-catenin transactivating complex IEA Reactome
Developmental Biology IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent nuclear events downstream of ESR-membrane signaling IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
G2/M Transition IEA Reactome
Heme signaling IEA Reactome
HuR (ELAVL1) binds and stabilizes mRNA IEA Reactome
M Phase IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Metabolism of RNA IEA Reactome
MITF-M-regulated melanocyte development IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Regulation of mRNA stability by proteins that bind AU-rich elements IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome
Transcriptional and post-translational regulation of MITF-M expression and activity IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here