Gene description for Vamp7
Gene name vesicle-associated membrane protein 7
Gene symbol Vamp7
Other names/aliases Sybl1
Species Rattus norvegicus
 Database cross references - Vamp7
ExoCarta ExoCarta_85491
Entrez Gene 85491
UniProt Q9JHW5  
 Vamp7 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Vamp7
Molecular Function
    SNAP receptor activity GO:0005484 IDA
    syntaxin binding GO:0019905 IPI
    protein binding GO:0005515 IPI
Biological Process
    calcium ion-dependent exocytosis GO:0017156 IDA
    phagocytosis, engulfment GO:0006911 IDA
    exocytosis GO:0006887 IDA
    vesicle transport along microtubule GO:0047496 IDA
    endosome to lysosome transport GO:0008333 ISO
    positive regulation of dendrite morphogenesis GO:0050775 ISO
    ER to Golgi vesicle-mediated transport GO:0006888 IDA
    regulation of protein targeting to vacuolar membrane GO:1900483 ISO
    neutrophil degranulation GO:0043312 ISO
    SNARE complex assembly GO:0035493 ISO
    Golgi to plasma membrane protein transport GO:0043001 ISO
    vesicle fusion GO:0006906 ISO
    autophagosome fusion GO:0000046 ISO
    eosinophil degranulation GO:0043308 ISO
    vesicle fusion with Golgi apparatus GO:0048280 IDA
    positive regulation of histamine secretion by mast cell GO:1903595 ISO
    natural killer cell degranulation GO:0043320 ISO
    vesicle-mediated transport GO:0016192 ISO
    triglyceride transport GO:0034197 IMP
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    lamellipodium GO:0030027 ISO
    late endosome membrane GO:0031902 IDA
    cell surface GO:0009986 ISO
    neuron projection GO:0043005 ISS
    phagocytic vesicle membrane GO:0030670 IEA
    azurophil granule membrane GO:0035577 ISO
    secretory granule membrane GO:0030667 ISO
    cell junction GO:0030054 IEA
    integral component of membrane GO:0016021 IEA
    SNARE complex GO:0031201 ISO
    synapse GO:0045202 IEA
    secretory granule GO:0030141 ISO
    pseudopodium GO:0031143 ISO
    intracellular GO:0005622 ISO
    apical part of cell GO:0045177 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    membrane GO:0016020 ISO
    lysosomal membrane GO:0005765 ISO
    trans-Golgi network GO:0005802 ISO
    platelet alpha granule GO:0031091 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISO
    transport vesicle membrane GO:0030658 IEA
    cell GO:0005623 ISO
    Golgi apparatus GO:0005794 ISO
    transport vesicle GO:0030133 ISO
    extracellular exosome GO:0070062 ISO
    phagocytic vesicle GO:0045335 ISS
 Experiment description of studies that identified Vamp7 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Vamp7
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vamp7 is involved
PathwayEvidenceSource
Clathrin derived vesicle budding IEA Reactome
Golgi Associated Vesicle Biogenesis IEA Reactome
Lysosome Vesicle Biogenesis IEA Reactome





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