Gene description for Vamp7
Gene name vesicle-associated membrane protein 7
Gene symbol Vamp7
Other names/aliases Sybl1
Species Rattus norvegicus
 Database cross references - Vamp7
ExoCarta ExoCarta_85491
Vesiclepedia VP_85491
Entrez Gene 85491
UniProt Q9JHW5  
 Vamp7 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Vamp7
Molecular Function
    SNARE binding GO:0000149 IBA
    SNAP receptor activity GO:0005484 IBA
    SNAP receptor activity GO:0005484 IDA
    protein binding GO:0005515 IPI
    syntaxin binding GO:0019905 IPI
Biological Process
    exocytosis GO:0006887 IBA
    exocytosis GO:0006887 IDA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IDA
    vesicle fusion GO:0006906 IBA
    vesicle fusion GO:0006906 IDA
    vesicle fusion GO:0006906 ISO
    phagocytosis, engulfment GO:0006911 IDA
    endosome to lysosome transport GO:0008333 IBA
    endosome to lysosome transport GO:0008333 IDA
    endosome to lysosome transport GO:0008333 ISO
    synaptic vesicle exocytosis GO:0016079 EXP
    synaptic vesicle exocytosis GO:0016079 IDA
    synaptic vesicle exocytosis GO:0016079 IMP
    vesicle-mediated transport GO:0016192 IDA
    vesicle-mediated transport GO:0016192 ISO
    calcium-ion regulated exocytosis GO:0017156 IDA
    triglyceride transport GO:0034197 IMP
    SNARE complex assembly GO:0035493 ISO
    Golgi to plasma membrane protein transport GO:0043001 ISO
    eosinophil degranulation GO:0043308 IDA
    eosinophil degranulation GO:0043308 ISO
    neutrophil degranulation GO:0043312 IDA
    neutrophil degranulation GO:0043312 ISO
    natural killer cell degranulation GO:0043320 ISO
    vesicle transport along microtubule GO:0047496 IDA
    vesicle fusion with Golgi apparatus GO:0048280 IDA
    positive regulation of dendrite morphogenesis GO:0050775 ISO
    autophagosome maturation GO:0097352 ISO
    regulation of protein targeting to vacuolar membrane GO:1900483 ISO
    positive regulation of histamine secretion by mast cell GO:1903595 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    lysosomal membrane GO:0005765 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 ISO
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    membrane GO:0016020 ISO
    lamellipodium GO:0030027 ISO
    transport vesicle GO:0030133 ISO
    secretory granule GO:0030141 ISO
    filopodium GO:0030175 IDA
    secretory granule membrane GO:0030667 ISO
    phagocytic vesicle membrane GO:0030670 IEA
    synaptic vesicle membrane GO:0030672 IDA
    platelet alpha granule GO:0031091 ISO
    pseudopodium GO:0031143 ISO
    SNARE complex GO:0031201 IBA
    SNARE complex GO:0031201 IDA
    SNARE complex GO:0031201 ISO
    late endosome membrane GO:0031902 IDA
    azurophil granule membrane GO:0035577 ISO
    neuron projection GO:0043005 IDA
    neuron projection GO:0043005 ISO
    neuron projection GO:0043005 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    apical part of cell GO:0045177 ISO
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    hippocampal mossy fiber to CA3 synapse GO:0098686 IDA
 Experiment description of studies that identified Vamp7 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Vamp7
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here