Gene description for Abcf1
Gene name ATP-binding cassette, subfamily F (GCN20), member 1
Gene symbol Abcf1
Other names/aliases Abc50
Species Rattus norvegicus
 Database cross references - Abcf1
ExoCarta ExoCarta_85493
Vesiclepedia VP_85493
Entrez Gene 85493
UniProt Q6MG08  
 Abcf1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Abcf1
Molecular Function
    ATP binding GO:0005524 IBA
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    translation activator activity GO:0008494 ISO
    translation activator activity GO:0008494 ISS
    ATP hydrolysis activity GO:0016887 IEA
    ribosome binding GO:0043022 ISO
    ribosome binding GO:0043022 ISS
Biological Process
    translation GO:0006412 NAS
    translational initiation GO:0006413 ISO
    translational initiation GO:0006413 ISS
    ribosome biogenesis GO:0042254 ISO
    ribosome biogenesis GO:0042254 ISS
    positive regulation of translation GO:0045727 ISO
    positive regulation of translation GO:0045727 ISS
Subcellular Localization
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    ribosome GO:0005840 IDA
 Experiment description of studies that identified Abcf1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Abcf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Abcf1 is involved
PathwayEvidenceSource
ABC-family proteins mediated transport IEA Reactome
Transport of small molecules IEA Reactome





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