Gene description for ABCB11
Gene name ATP-binding cassette, sub-family B (MDR/TAP), member 11
Gene symbol ABCB11
Other names/aliases ABC16
BRIC2
BSEP
PFIC-2
PFIC2
PGY4
SPGP
Species Homo sapiens
 Database cross references - ABCB11
ExoCarta ExoCarta_8647
Vesiclepedia VP_8647
Entrez Gene 8647
HGNC 42
MIM 603201
UniProt O95342  
 ABCB11 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 19056867    
 Gene ontology annotations for ABCB11
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ABC-type xenobiotic transporter activity GO:0008559 IMP
    ABC-type xenobiotic transporter activity GO:0008559 ISS
    bile acid transmembrane transporter activity GO:0015125 IMP
    bile acid transmembrane transporter activity GO:0015125 ISS
    canalicular bile acid transmembrane transporter activity GO:0015126 IBA
    canalicular bile acid transmembrane transporter activity GO:0015126 IDA
    canalicular bile acid transmembrane transporter activity GO:0015126 IMP
    canalicular bile acid transmembrane transporter activity GO:0015126 ISS
    carbohydrate transmembrane transporter activity GO:0015144 IEA
    ABC-type bile acid transporter activity GO:0015432 IBA
    ABC-type bile acid transporter activity GO:0015432 IDA
    ABC-type bile acid transporter activity GO:0015432 IMP
    ABC-type bile acid transporter activity GO:0015432 ISS
    ABC-type bile acid transporter activity GO:0015432 TAS
    ATP hydrolysis activity GO:0016887 IEA
Biological Process
    fatty acid metabolic process GO:0006631 ISS
    bile acid biosynthetic process GO:0006699 TAS
    xenobiotic metabolic process GO:0006805 IMP
    xenobiotic metabolic process GO:0006805 ISS
    xenobiotic transmembrane transport GO:0006855 IMP
    xenobiotic transmembrane transport GO:0006855 ISS
    response to oxidative stress GO:0006979 IEA
    bile acid metabolic process GO:0008206 IDA
    bile acid metabolic process GO:0008206 IMP
    bile acid metabolic process GO:0008206 ISS
    response to organic cyclic compound GO:0014070 IEA
    bile acid and bile salt transport GO:0015721 IDA
    bile acid and bile salt transport GO:0015721 IMP
    bile acid and bile salt transport GO:0015721 ISS
    bile acid and bile salt transport GO:0015721 TAS
    canalicular bile acid transport GO:0015722 IBA
    canalicular bile acid transport GO:0015722 IDA
    canalicular bile acid transport GO:0015722 IMP
    canalicular bile acid transport GO:0015722 ISS
    protein ubiquitination GO:0016567 IMP
    protein ubiquitination GO:0016567 ISS
    regulation of fatty acid beta-oxidation GO:0031998 ISS
    carbohydrate transmembrane transport GO:0034219 IEA
    cholesterol homeostasis GO:0042632 ISS
    response to estrogen GO:0043627 IEA
    response to ethanol GO:0045471 IEA
    xenobiotic export from cell GO:0046618 IMP
    xenobiotic export from cell GO:0046618 ISS
    transmembrane transport GO:0055085 IBA
    lipid homeostasis GO:0055088 ISS
    phospholipid homeostasis GO:0055091 ISS
    positive regulation of bile acid secretion GO:0120189 ISS
    regulation of bile acid metabolic process GO:1904251 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    endosome GO:0005768 ISS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IMP
    basolateral plasma membrane GO:0016323 ISS
    apical plasma membrane GO:0016324 IBA
    apical plasma membrane GO:0016324 IMP
    apical plasma membrane GO:0016324 ISS
    intercellular canaliculus GO:0046581 IEA
    intracellular canaliculus GO:0046691 ISS
    recycling endosome GO:0055037 ISS
    recycling endosome membrane GO:0055038 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ABCB11 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for ABCB11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ap2a1  
Reconstituted Complex Rattus norvegicus
2 AP2A1 160
Affinity Capture-Western Homo sapiens
3 LY6D 8581
Two-hybrid Homo sapiens
4 NR4A1  
Two-hybrid Homo sapiens
5 Ap2m1 116563
Reconstituted Complex Rattus norvegicus
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