Gene description for MGAM
Gene name maltase-glucoamylase
Gene symbol MGAM
Other names/aliases MG
MGA
Species Homo sapiens
 Database cross references - MGAM
ExoCarta ExoCarta_8972
Entrez Gene 8972
HGNC 7043
MIM 154360
UniProt O43451  
 MGAM identified in exosomes derived from the following tissue/cell type
Urine 15326289    
Urine 22418980    
Urine 22418980    
Urine 25452312    
 Gene ontology annotations for MGAM
Molecular Function
    carbohydrate binding GO:0030246 IEA
    amylase activity GO:0016160 IEA
    glucan 1,4-alpha-glucosidase activity GO:0004339 IEA
    maltose alpha-glucosidase activity GO:0032450 IEA
    catalytic activity GO:0003824 TAS
    alpha-1,4-glucosidase activity GO:0004558 TAS
Biological Process
    small molecule metabolic process GO:0044281 TAS
    maltose metabolic process GO:0000023 IEA
    starch catabolic process GO:0005983 TAS
    carbohydrate metabolic process GO:0005975 TAS
    polysaccharide digestion GO:0044245 TAS
Subcellular Localization
    apical plasma membrane GO:0016324 IEA
    extracellular exosome GO:0070062 IDA
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified MGAM in exosomes
1
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 238
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R.
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MGAM
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MGAM is involved
PathwayEvidenceSource
Digestion of dietary carbohydrate TAS Reactome





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