Gene description for Vamp5
Gene name vesicle-associated membrane protein 5
Gene symbol Vamp5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Vamp5
ExoCarta ExoCarta_89818
Entrez Gene 89818
UniProt Q9Z2J5  
 Vamp5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Vamp5
Molecular Function
    SNAP receptor activity GO:0005484 IBA
    SNARE binding GO:0000149 IBA
Biological Process
    Golgi to plasma membrane protein transport GO:0043001 ISO
    vesicle fusion GO:0006906 IBA
    exocytosis GO:0006887 IBA
    skeletal muscle tissue development GO:0007519 ISO
    cell differentiation GO:0030154 IEA
Subcellular Localization
    intercalated disc GO:0014704 ISO
    plasma membrane GO:0005886 ISO
    cytoplasmic vesicle membrane GO:0030659 ISO
    late endosome GO:0005770 ISO
    cell surface GO:0009986 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    SNARE complex GO:0031201 IBA
    extracellular exosome GO:0070062 ISO
    intracellular GO:0005622 ISO
    integral component of organelle membrane GO:0031301 ISO
    trans-Golgi network GO:0005802 IDA
    integral component of plasma membrane GO:0005887 ISO
    synaptic vesicle GO:0008021 IBA
    cell GO:0005623 ISO
 Experiment description of studies that identified Vamp5 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Vamp5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Vamp5 is involved
No pathways found





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