Gene description for KRT75
Gene name keratin 75, type II
Gene symbol KRT75
Other names/aliases K6HF
KB18
PFB
Species Homo sapiens
 Database cross references - KRT75
ExoCarta ExoCarta_9119
Vesiclepedia VP_9119
Entrez Gene 9119
HGNC 24431
MIM 609025
UniProt O95678  
 KRT75 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 19837982    
Neuroblastoma cells 25944692    
Prostate cancer cells 22723089    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KRT75
Molecular Function
    structural molecule activity GO:0005198 TAS
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cornified envelope GO:0001533 IEA
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 IDA
    keratin filament GO:0045095 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT75 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
8
Experiment ID 21
MISEV standards
EM|IEM
Biophysical techniques
Alix|TSG101|HSP70|CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 146
MISEV standards
EM
Biophysical techniques
HSP70|HSP90|CD9
Enriched markers
HSP90B1
Negative markers
Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name RWPE - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
11
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KRT75
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT76 51350
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
2 KRT77 374454
Cross-Linking-MS (XL-MS) Homo sapiens
3 SUFU 51684
Two-hybrid Homo sapiens
4 KRT37 8688
Two-hybrid Homo sapiens
5 KRT73 319101
Cross-Linking-MS (XL-MS) Homo sapiens
6 EPM2AIP1 9852
Two-hybrid Homo sapiens
7 LGR4 55366
Affinity Capture-MS Homo sapiens
8 TRIML2  
Two-hybrid Homo sapiens
9 CPNE7 27132
Two-hybrid Homo sapiens
10 KIFC3 3801
Two-hybrid Homo sapiens
11 SCNM1  
Two-hybrid Homo sapiens
12 KRT17 3872
Cross-Linking-MS (XL-MS) Homo sapiens
13 CARD9 64170
Two-hybrid Homo sapiens
14 MYO15B 80022
Two-hybrid Homo sapiens
15 MNS1 55329
Two-hybrid Homo sapiens
16 CCHCR1  
Two-hybrid Homo sapiens
17 EPS15 2060
Affinity Capture-MS Homo sapiens
18 TRIM27  
Two-hybrid Homo sapiens
19 CAND1 55832
Affinity Capture-MS Homo sapiens
20 RNF208  
Affinity Capture-MS Homo sapiens
21 CCDC102B  
Two-hybrid Homo sapiens
22 STX11 8676
Two-hybrid Homo sapiens
23 TRIM54  
Two-hybrid Homo sapiens
24 MYOZ3  
Two-hybrid Homo sapiens
25 KCTD7 154881
Two-hybrid Homo sapiens
26 LMO2  
Two-hybrid Homo sapiens
27 PLEKHG4  
Two-hybrid Homo sapiens
28 CRACR2A  
Two-hybrid Homo sapiens
29 CEMIP 57214
Affinity Capture-MS Homo sapiens
30 NME7 29922
Two-hybrid Homo sapiens
31 COPS6 10980
Affinity Capture-MS Homo sapiens
32 C19orf25  
Two-hybrid Homo sapiens
33 HGS 9146
Two-hybrid Homo sapiens
34 COPS5 10987
Affinity Capture-MS Homo sapiens
35 FBXO17 115290
Two-hybrid Homo sapiens
36 KRT40  
Two-hybrid Homo sapiens
37 MAGOHB 55110
Two-hybrid Homo sapiens
38 CCDC53 51019
Two-hybrid Homo sapiens
39 OIP5  
Two-hybrid Homo sapiens
40 MTUS2 23281
Two-hybrid Homo sapiens
41 KRT12 3859
Cross-Linking-MS (XL-MS) Homo sapiens
42 U2AF1 7307
Two-hybrid Homo sapiens
43 KRT72 140807
Cross-Linking-MS (XL-MS) Homo sapiens
44 CEP57  
Two-hybrid Homo sapiens
45 ZWINT  
Two-hybrid Homo sapiens
46 CUL1 8454
Affinity Capture-MS Homo sapiens
47 TFCP2 7024
Affinity Capture-MS Homo sapiens
48 PMPCB 9512
Affinity Capture-MS Homo sapiens
49 RCOR3  
Two-hybrid Homo sapiens
50 LMO4  
Two-hybrid Homo sapiens
51 KRT25 147183
Two-hybrid Homo sapiens
52 C1orf216  
Two-hybrid Homo sapiens
53 GOLGA6L9  
Two-hybrid Homo sapiens
54 NFKBID  
Two-hybrid Homo sapiens
55 CXXC1  
Affinity Capture-MS Homo sapiens
56 LONRF1  
Two-hybrid Homo sapiens
57 WASH1 100287171
Two-hybrid Homo sapiens
58 KRT34  
Two-hybrid Homo sapiens
59 BEX2  
Two-hybrid Homo sapiens
60 SPTA1  
Two-hybrid Homo sapiens
61 PRPH 5630
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
62 CUL4B 8450
Affinity Capture-MS Homo sapiens
63 GOLGA2 2801
Two-hybrid Homo sapiens
64 KRT35 3886
Two-hybrid Homo sapiens
65 KRT16 3868
Cross-Linking-MS (XL-MS) Homo sapiens
66 TNPO2 30000
Two-hybrid Homo sapiens
67 TSSK3  
Two-hybrid Homo sapiens
68 TCHP  
Two-hybrid Homo sapiens
69 IQUB  
Two-hybrid Homo sapiens
70 KRT14 3861
Cross-Linking-MS (XL-MS) Homo sapiens
71 DYDC1  
Two-hybrid Homo sapiens
72 KRT2 3849
Cross-Linking-MS (XL-MS) Homo sapiens
73 CUL2 8453
Affinity Capture-MS Homo sapiens
74 YAP1 10413
Affinity Capture-MS Homo sapiens
75 KRT79 338785
Cross-Linking-MS (XL-MS) Homo sapiens
76 C6orf165  
Two-hybrid Homo sapiens
77 TEKT1  
Two-hybrid Homo sapiens
78 NUMA1 4926
Affinity Capture-MS Homo sapiens
79 GAN 8139
Affinity Capture-MS Homo sapiens
80 KRT3 3850
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
81 KRT33B 3884
Two-hybrid Homo sapiens
82 ASB15 142685
Two-hybrid Homo sapiens
83 KANK2 25959
Two-hybrid Homo sapiens
84 LCA5L  
Two-hybrid Homo sapiens
85 Kifc1  
Affinity Capture-MS Mus musculus
86 TEKT4  
Two-hybrid Homo sapiens
87 TXLNB  
Two-hybrid Homo sapiens
88 EPPK1 83481
Affinity Capture-MS Homo sapiens
89 ZC4H2 55906
Affinity Capture-MS Homo sapiens
90 CUL3 8452
Affinity Capture-MS Homo sapiens
91 DES 1674
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
92 AMOT  
Two-hybrid Homo sapiens
93 KRT26 353288
Two-hybrid Homo sapiens
94 PM20D2 135293
Two-hybrid Homo sapiens
95 KRT27 342574
Two-hybrid Homo sapiens
96 KRT15 3866
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
97 GPANK1  
Two-hybrid Homo sapiens
98 XPOT 11260
Two-hybrid Homo sapiens
99 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
100 NUDT8  
Affinity Capture-MS Homo sapiens
101 GLYCTK  
Two-hybrid Homo sapiens
102 TJAP1 93643
Two-hybrid Homo sapiens
103 KRT84 3890
Cross-Linking-MS (XL-MS) Homo sapiens
104 USHBP1  
Two-hybrid Homo sapiens
105 CDKN2D  
Two-hybrid Homo sapiens
106 KRT19 3880
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
107 NUP62 23636
Two-hybrid Homo sapiens
108 ALS2CR11  
Two-hybrid Homo sapiens
109 LINC00521  
Two-hybrid Homo sapiens
110 DHX15 1665
Two-hybrid Homo sapiens
111 NUDT21 11051
Two-hybrid Homo sapiens
112 BCAS2 10286
Two-hybrid Homo sapiens
113 KRT36 8689
Two-hybrid Homo sapiens
114 NEK6 10783
Two-hybrid Homo sapiens
115 WWOX 51741
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
116 ARMC7  
Two-hybrid Homo sapiens
117 POLR3C 10623
Two-hybrid Homo sapiens
118 POLR1C 9533
Two-hybrid Homo sapiens
119 KRT10 3858
Cross-Linking-MS (XL-MS) Homo sapiens
120 INCA1  
Two-hybrid Homo sapiens
121 CDC37 11140
Two-hybrid Homo sapiens
122 CUL5 8065
Affinity Capture-MS Homo sapiens
123 CDK18 5129
Two-hybrid Homo sapiens
124 LOC102724594 102724594
Two-hybrid Homo sapiens
125 MKRN3  
Two-hybrid Homo sapiens
126 DMAP1 55929
Two-hybrid Homo sapiens
127 KRT71 112802
Cross-Linking-MS (XL-MS) Homo sapiens
128 EIF4A3 9775
Affinity Capture-MS Homo sapiens
129 OSGIN1  
Two-hybrid Homo sapiens
130 Nup214  
Affinity Capture-MS Mus musculus
131 KRT24 192666
Two-hybrid Homo sapiens
132 ABI2 10152
Two-hybrid Homo sapiens
133 KRT20 54474
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
134 KRT38 8687
Two-hybrid Homo sapiens
135 TRIM23 373
Two-hybrid Homo sapiens
136 SORT1 6272
Affinity Capture-MS Homo sapiens
137 C1orf109  
Two-hybrid Homo sapiens
138 NEDD8 4738
Affinity Capture-MS Homo sapiens
139 HNRNPF 3185
Two-hybrid Homo sapiens
140 KRT13 3860
Two-hybrid Homo sapiens
141 LMO1  
Two-hybrid Homo sapiens
142 VIM 7431
Cross-Linking-MS (XL-MS) Homo sapiens
143 KRT7 3855
Cross-Linking-MS (XL-MS) Homo sapiens
144 CUL4A 8451
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KRT75 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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