Gene description for KL
Gene name klotho
Gene symbol KL
Other names/aliases -
Species Homo sapiens
 Database cross references - KL
ExoCarta ExoCarta_9365
Vesiclepedia VP_9365
Entrez Gene 9365
HGNC 6344
MIM 604824
UniProt Q9UEF7  
 KL identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Urine 19056867    
 Gene ontology annotations for KL
Molecular Function
    beta-glucuronidase activity GO:0004566 IEA
    fibroblast growth factor receptor binding GO:0005104 IBA
    hormone activity GO:0005179 IEA
    vitamin D binding GO:0005499 IEA
    beta-glucosidase activity GO:0008422 TAS
    fibroblast growth factor binding GO:0017134 IBA
    fibroblast growth factor binding GO:0017134 IPI
Biological Process
    negative regulation of systemic arterial blood pressure GO:0003085 IEA
    carbohydrate metabolic process GO:0005975 IEA
    energy reserve metabolic process GO:0006112 IEA
    determination of adult lifespan GO:0008340 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 IBA
    response to activity GO:0014823 IEA
    positive regulation of bone mineralization GO:0030501 IMP
    response to vitamin D GO:0033280 IEA
    norepinephrine biosynthetic process GO:0042421 IEA
    calcium ion homeostasis GO:0055074 IEA
    positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway GO:0090080 IEA
    response to angiotensin GO:1990776 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 TAS
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 TAS
    apical plasma membrane GO:0016324 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KL in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for KL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MDM2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 SHANK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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