Gene description for S100a9
Gene name S100 calcium binding protein A9
Gene symbol S100a9
Other names/aliases Mrp14
Species Rattus norvegicus
 Database cross references - S100a9
ExoCarta ExoCarta_94195
Vesiclepedia VP_94195
Entrez Gene 94195
UniProt P50116  
 S100a9 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for S100a9
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IEA
    antioxidant activity GO:0016209 IEA
    calcium-dependent protein binding GO:0048306 IBA
Biological Process
    leukocyte migration involved in inflammatory response GO:0002523 IBA
    leukocyte migration involved in inflammatory response GO:0002523 IEA
    leukocyte migration involved in inflammatory response GO:0002523 ISO
    leukocyte migration involved in inflammatory response GO:0002523 ISS
    chronic inflammatory response GO:0002544 IBA
    chronic inflammatory response GO:0002544 IEP
    peptide secretion GO:0002790 ISO
    positive regulation of peptide secretion GO:0002793 ISO
    regulation of translation GO:0006417 ISO
    autophagy GO:0006914 IEA
    autophagy GO:0006914 ISO
    autophagy GO:0006914 ISS
    apoptotic process GO:0006915 IEA
    response to zinc ion GO:0010043 IEP
    positive regulation of neuron projection development GO:0010976 IDA
    astrocyte development GO:0014002 IBA
    astrocyte development GO:0014002 ISO
    peptidyl-cysteine S-nitrosylation GO:0018119 ISO
    actin cytoskeleton organization GO:0030036 ISO
    positive regulation of blood coagulation GO:0030194 ISO
    neutrophil chemotaxis GO:0030593 IBA
    neutrophil chemotaxis GO:0030593 IEA
    neutrophil chemotaxis GO:0030593 ISO
    neutrophil chemotaxis GO:0030593 ISS
    leukocyte chemotaxis GO:0030595 ISO
    response to lipopolysaccharide GO:0032496 IEP
    autocrine signaling GO:0035425 IBA
    autocrine signaling GO:0035425 IGI
    peptidyl-cysteine S-trans-nitrosylation GO:0035606 ISO
    modulation of process of another organism GO:0035821 IEA
    endothelial cell migration GO:0043542 IBA
    innate immune response GO:0045087 IEA
    regulation of integrin biosynthetic process GO:0045113 ISO
    response to ethanol GO:0045471 IEP
    positive regulation of inflammatory response GO:0050729 IEA
    positive regulation of inflammatory response GO:0050729 ISO
    positive regulation of inflammatory response GO:0050729 ISS
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IBA
    antimicrobial humoral immune response mediated by antimicrobial peptide GO:0061844 IEA
    neutrophil aggregation GO:0070488 IBA
    neutrophil aggregation GO:0070488 IEA
    neutrophil aggregation GO:0070488 ISO
    neutrophil aggregation GO:0070488 ISS
    cellular oxidant detoxification GO:0098869 IEA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISO
    positive regulation of cysteine-type endopeptidase activity GO:2001056 ISS
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 IEA
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISS
Subcellular Localization
    cornified envelope GO:0001533 ISO
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    calprotectin complex GO:1990660 IEA
    calprotectin complex GO:1990660 ISO
    S100A9 complex GO:1990662 IEA
    S100A9 complex GO:1990662 ISO
 Experiment description of studies that identified S100a9 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for S100a9
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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