Gene description for Rab14
Gene name RAB14, member RAS oncogene family
Gene symbol Rab14
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rab14
ExoCarta ExoCarta_94197
Vesiclepedia VP_94197
Entrez Gene 94197
UniProt P61107  
 Rab14 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rab14
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    GTPase activity GO:0003924 NAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
Biological Process
    phagolysosome assembly GO:0001845 IBA
    intracellular protein transport GO:0006886 IBA
    Golgi to endosome transport GO:0006895 IBA
    Golgi to endosome transport GO:0006895 IEA
    Golgi to endosome transport GO:0006895 ISO
    Golgi to endosome transport GO:0006895 ISS
    Golgi to endosome transport GO:0006895 TAS
    body fluid secretion GO:0007589 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 ISO
    fibroblast growth factor receptor signaling pathway GO:0008543 ISS
    vesicle-mediated transport GO:0016192 NAS
    endocytic recycling GO:0032456 IEA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    regulation of protein localization GO:0032880 IDA
    regulation of protein localization GO:0032880 IEA
    regulation of protein localization GO:0032880 IMP
    regulation of protein localization GO:0032880 ISO
    endosomal vesicle fusion GO:0034058 IEA
    endosomal vesicle fusion GO:0034058 ISO
    early endosome to Golgi transport GO:0034498 IEA
    early endosome to Golgi transport GO:0034498 ISO
    defense response to bacterium GO:0042742 IEA
    apical protein localization GO:0045176 IDA
    regulation of embryonic development GO:0045995 IEA
    regulation of embryonic development GO:0045995 ISO
    regulation of embryonic development GO:0045995 ISS
    intracellular transport GO:0046907 NAS
    phagosome maturation GO:0090382 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    lysosome GO:0005764 IDA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    rough endoplasmic reticulum GO:0005791 IDA
    Golgi stack GO:0005795 IDA
    trans-Golgi network GO:0005802 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IDA
    cilium GO:0005929 ISO
    endomembrane system GO:0012505 IBA
    apical plasma membrane GO:0016324 IDA
    trans-Golgi network transport vesicle GO:0030140 IDA
    cytoplasmic vesicle membrane GO:0030659 TAS
    synaptic vesicle membrane GO:0030672 EXP
    synaptic vesicle membrane GO:0030672 IDA
    synaptic vesicle membrane GO:0030672 IEA
    synaptic vesicle membrane GO:0030672 IMP
    early endosome membrane GO:0031901 IEA
    early endosome membrane GO:0031901 ISO
    nuclear outer membrane-endoplasmic reticulum membrane network GO:0042175 IDA
    intracellular membrane-bounded organelle GO:0043231 ISO
    phagocytic vesicle GO:0045335 IBA
    phagocytic vesicle GO:0045335 IEA
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    perinuclear region of cytoplasm GO:0048471 IDA
    recycling endosome GO:0055037 IEA
    recycling endosome GO:0055037 ISO
    recycling endosome GO:0055037 ISS
    alveolar lamellar body GO:0097208 IDA
 Experiment description of studies that identified Rab14 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rab14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ocrl  
Two-hybrid Rattus norvegicus
2 Sumo3  
Affinity Capture-MS Rattus norvegicus
3 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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