ExoCarta: Gene summary

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Gene description for Psmb1
Gene name	proteasome (prosome, macropain) subunit, beta type 1
Gene symbol	Psmb1
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Psmb1
ExoCarta	ExoCarta_94198
Vesiclepedia	VP_94198
Entrez Gene	94198
UniProt	P18421
Psmb1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Psmb1

Molecular Function
	endopeptidase activity	GO:0004175	IBA
	threonine-type endopeptidase activity	GO:0004298	IEA
Biological Process
	proteasome-mediated ubiquitin-dependent protein catabolic process	GO:0043161	IBA
	proteasomal ubiquitin-independent protein catabolic process	GO:0010499	IBA
Subcellular Localization
	proteasome complex	GO:0000502	ISO
	cytoplasm	GO:0005737	IBA
	nucleus	GO:0005634	ISO
	extracellular exosome	GO:0070062	ISO
	proteasome core complex	GO:0005839	ISS

Experiment description of studies that identified Psmb1 in exosomes

Experiment ID

MISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	EV Enriched markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting