Gene description for Rps27
Gene name ribosomal protein S27
Gene symbol Rps27
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rps27
ExoCarta ExoCarta_94266
Vesiclepedia VP_94266
Entrez Gene 94266
UniProt Q71TY3  
 Rps27 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rps27
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    ribosomal small subunit assembly GO:0000028 IBA
    rRNA processing GO:0006364 ISO
    rRNA processing GO:0006364 ISS
    translation GO:0006412 IEA
    biological_process GO:0008150 ND
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
    translation at presynapse GO:0140236 ISO
    translation at postsynapse GO:0140242 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    cytosol GO:0005829 IEA
    ribosome GO:0005840 IEA
    ribosome GO:0005840 ISO
    postsynaptic density GO:0014069 ISO
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    synapse GO:0045202 ISO
    presynapse GO:0098793 EXP
    presynapse GO:0098793 IDA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 EXP
    glutamatergic synapse GO:0098978 IDA
    GABA-ergic synapse GO:0098982 EXP
    GABA-ergic synapse GO:0098982 IDA
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified Rps27 in exosomes
1
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rps27
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tollip 361677
Two-hybrid Rattus norvegicus
View the network image/svg+xml
 Pathways in which Rps27 is involved
PathwayEvidenceSource
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Cap-dependent Translation Initiation IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
Eukaryotic Translation Initiation IEA Reactome
Formation of a pool of free 40S subunits IEA Reactome
Formation of the ternary complex, and subsequently, the 43S complex IEA Reactome
GTP hydrolysis and joining of the 60S ribosomal subunit IEA Reactome
L13a-mediated translational silencing of Ceruloplasmin expression IEA Reactome
M Phase IEA Reactome
Major pathway of rRNA processing in the nucleolus and cytosol IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) IEA Reactome
Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) IEA Reactome
Nonsense-Mediated Decay (NMD) IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Ribosomal scanning and start codon recognition IEA Reactome
rRNA processing IEA Reactome
rRNA processing in the nucleus and cytosol IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
Translation IEA Reactome
Translation initiation complex formation IEA Reactome





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