Gene description for Atp1a2
Gene name ATPase, Na+/K+ transporting, alpha 2 polypeptide
Gene symbol Atp1a2
Other names/aliases AW060654
Atpa-3
mKIAA0778
Species Mus musculus
 Database cross references - Atp1a2
ExoCarta ExoCarta_98660
Vesiclepedia VP_98660
Entrez Gene 98660
UniProt Q6PIE5  
 Atp1a2 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Atp1a2
Molecular Function
    P-type sodium:potassium-exchanging transporter activity GO:0005391 IBA
    P-type sodium:potassium-exchanging transporter activity GO:0005391 ISO
    P-type sodium:potassium-exchanging transporter activity GO:0005391 ISS
    steroid binding GO:0005496 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP hydrolysis activity GO:0016887 IEA
    ATP hydrolysis activity GO:0016887 ISO
    ATPase-coupled monoatomic cation transmembrane transporter activity GO:0019829 ISO
    potassium ion binding GO:0030955 IEA
    potassium ion binding GO:0030955 ISO
    sodium ion binding GO:0031402 IEA
    sodium ion binding GO:0031402 ISO
    protein heterodimerization activity GO:0046982 IEA
    protein heterodimerization activity GO:0046982 ISO
    protein-folding chaperone binding GO:0051087 IEA
    protein-folding chaperone binding GO:0051087 ISO
    steroid hormone binding GO:1990239 IEA
    steroid hormone binding GO:1990239 ISO
Biological Process
    neurotransmitter uptake GO:0001504 IMP
    neurotransmitter uptake GO:0001504 IMP
    behavioral fear response GO:0001662 IMP
    regulation of the force of heart contraction GO:0002026 IMP
    regulation of respiratory gaseous exchange by nervous system process GO:0002087 IMP
    potassium ion transport GO:0006813 ISO
    sodium ion transport GO:0006814 ISO
    intracellular sodium ion homeostasis GO:0006883 IBA
    intracellular sodium ion homeostasis GO:0006883 IEA
    intracellular sodium ion homeostasis GO:0006883 ISO
    regulation of muscle contraction GO:0006937 IMP
    regulation of smooth muscle contraction GO:0006940 IMP
    regulation of striated muscle contraction GO:0006942 IMP
    blood circulation GO:0008015 IGI
    regulation of blood pressure GO:0008217 IGI
    adult locomotory behavior GO:0008344 IMP
    visual learning GO:0008542 IMP
    establishment or maintenance of transmembrane electrochemical gradient GO:0010248 IEA
    response to auditory stimulus GO:0010996 IMP
    neuronal action potential propagation GO:0019227 IMP
    regulation of vasoconstriction GO:0019229 IGI
    regulation of vasoconstriction GO:0019229 IMP
    L-ascorbic acid metabolic process GO:0019852 IGI
    amygdala development GO:0021764 IMP
    olfactory cortex development GO:0021989 IMP
    intracellular potassium ion homeostasis GO:0030007 IBA
    intracellular potassium ion homeostasis GO:0030007 IEA
    intracellular potassium ion homeostasis GO:0030007 ISO
    response to nicotine GO:0035094 IEA
    response to nicotine GO:0035094 ISO
    locomotory exploration behavior GO:0035641 IMP
    sodium ion transmembrane transport GO:0035725 ISO
    response to potassium ion GO:0035864 IMP
    sodium ion export across plasma membrane GO:0036376 IBA
    sodium ion export across plasma membrane GO:0036376 IEA
    sodium ion export across plasma membrane GO:0036376 ISO
    locomotion GO:0040011 IMP
    fear response GO:0042596 IMP
    negative regulation of heart contraction GO:0045822 IMP
    negative regulation of striated muscle contraction GO:0045988 IMP
    ATP metabolic process GO:0046034 IEA
    ATP metabolic process GO:0046034 ISO
    negative regulation of cytosolic calcium ion concentration GO:0051481 IMP
    membrane depolarization GO:0051899 IMP
    cellular response to mechanical stimulus GO:0071260 IEA
    cellular response to mechanical stimulus GO:0071260 ISO
    cellular response to steroid hormone stimulus GO:0071383 IDA
    cellular response to steroid hormone stimulus GO:0071383 ISO
    potassium ion transmembrane transport GO:0071805 ISO
    regulation of cardiac muscle cell contraction GO:0086004 IEA
    regulation of cardiac muscle cell contraction GO:0086004 ISO
    monoatomic cation transmembrane transport GO:0098655 ISO
    proton transmembrane transport GO:1902600 IBA
    proton transmembrane transport GO:1902600 IEA
    proton transmembrane transport GO:1902600 ISO
    negative regulation of calcium ion transmembrane transport GO:1903170 IEA
    negative regulation of calcium ion transmembrane transport GO:1903170 ISO
    response to glycoside GO:1903416 IMP
    response to glycoside GO:1903416 ISO
    potassium ion import across plasma membrane GO:1990573 IBA
    potassium ion import across plasma membrane GO:1990573 IEA
    potassium ion import across plasma membrane GO:1990573 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    endoplasmic reticulum GO:0005783 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    sodium:potassium-exchanging ATPase complex GO:0005890 IBA
    sodium:potassium-exchanging ATPase complex GO:0005890 IEA
    sodium:potassium-exchanging ATPase complex GO:0005890 ISO
    caveola GO:0005901 IEA
    caveola GO:0005901 ISO
    cell surface GO:0009986 IDA
    intercalated disc GO:0014704 IEA
    intercalated disc GO:0014704 ISO
    membrane GO:0016020 ISO
    T-tubule GO:0030315 IDA
    T-tubule GO:0030315 ISO
    organelle membrane GO:0031090 IEA
    organelle membrane GO:0031090 ISO
    protein-containing complex GO:0032991 IDA
    sarcolemma GO:0042383 IDA
    sarcolemma GO:0042383 ISO
    cell projection GO:0042995 IBA
    cell projection GO:0042995 IDA
    neuronal cell body GO:0043025 IDA
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Atp1a2 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
3
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Atp1a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Vamp2  
Affinity Capture-MS Mus musculus
2 Slc8a1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
3 Sstr3  
Proximity Label-MS Mus musculus
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Usp7  
Affinity Capture-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Kcnma1  
Affinity Capture-MS Mus musculus
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