Description for Phosphatidylcholine
Lipid Phosphatidylcholine
Species Homo sapiens
 Phosphatidylcholine identified in sEVs derived from the following tissue/cell type
Astrocytes 25498500    
Faeces 33431899    
Faeces 33431899    
Glioblastoma cells 25498500    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
Prostate cancer cells 25519911    
 Experiment description of studies that identified Phosphatidylcholine in sEVs
1
Experiment ID 273
Identified molecule Lipid
Identification method Thin-layer chromatography
PubMed ID 25498500    
Organism Homo sapiens
Experiment description Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components.
Authors "Toda Y, Takata K, Nakagawa Y, Kawakami H, Fujioka S, Kobayashi K, Hattori Y, Kitamura Y, Akaji K, Ashihara E."
Journal name Biochem Biophys Res Commun
Publication year 2015
Sample Astrocytes
Sample name Astrocytes
Molecules identified in the study Lipids
Methods used in the study Thin layer chromatography
2
Experiment ID 305
Identified molecule Lipid
Identification method HPLC
PubMed ID 33431899    
Organism Homo sapiens
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Healthy volunteers
Molecules identified in the study Protein
Lipid
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
HPLC
3
Experiment ID 306
Identified molecule Lipid
Identification method HPLC
PubMed ID 33431899    
Organism Homo sapiens
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Type 2 diabetes patients
Molecules identified in the study Protein
Lipid
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
HPLC
4
Experiment ID 272
Identified molecule Lipid
Identification method Thin-layer chromatography
PubMed ID 25498500    
Organism Homo sapiens
Experiment description Effective internalization of U251-MG-secreted exosomes into cancer cells and characterization of their lipid components.
Authors "Toda Y, Takata K, Nakagawa Y, Kawakami H, Fujioka S, Kobayashi K, Hattori Y, Kitamura Y, Akaji K, Ashihara E."
Journal name Biochem Biophys Res Commun
Publication year 2015
Sample Glioblastoma cells
Sample name U251
Molecules identified in the study Lipids
Protein
Methods used in the study Thin layer chromatography
Western blotting
5
Experiment ID 245
Identified molecule Lipid
Identification method LC-MS
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - Ethanol treated (control)
Molecules identified in the study Lipids
Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 246
Identified molecule Lipid
Identification method LC-MS
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - sn-1-O-hexadecylglycerol treated
Molecules identified in the study Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 247
Identified molecule Lipid
Identification method LC-MS
PubMed ID 25519911    
Organism Homo sapiens
Experiment description The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells
Authors "Phuyal S, Skotland T, Hessvik NP, Simolin H, Overbye A, Brech A, Parton RG, Ekroos K, Sandvig K, Llorente A."
Journal name J Biol Chem
Publication year 2015
Sample Prostate cancer cells
Sample name PC-3 - DL- palmitin treated
Molecules identified in the study Lipids
Methods used in the study Western blotting
Mass spectrometry