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Results shown for selected experiment:

Experiment ID 16

PubMed ID 34108659

Identified molecule Protein

Species Homo sapiens

Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker

Sample Breast cancer cells

Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled

Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography

Methods used in the study Western blotting
Mass spectrometry

Flotation density -

Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."

Journal name Nat Cell Biol

Publication year 2021

   Contents identified in selected experiment:

   Top 200 abundant gene(s) for selected experiment:

   Other experiments from same study (PubMed ID):

PubMed ID Species Experiment description Sample Sample name Identifications Isolation methods Quantification

1 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography EXOQuant

2 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation EXOQuant

3 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography EXOQuant

4 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Breast cancer cells MDA-MB-231 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation EXOQuant

5 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Embryonic kidney cells HEK293T - Exo-rich fractions 7-10 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography EXOQuant

6 34108659 Homo sapiens Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker Pancreatic cancer cells PANC-1 - Exo-rich fractions 1-6 pooled Protein Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation EXOQuant

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	PubMed ID	Species	Experiment description	Sample	Sample name	Identifications	Isolation methods	Quantification
1	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Pancreatic cancer cells	PANC-1 - Exo-rich fractions 7-10 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography	EXOQuant
2	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Embryonic kidney cells	HEK293T - Exo-rich fractions 1-6 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation	EXOQuant
3	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Breast cancer cells	MDA-MB-231 - Exo-rich fractions 7-10 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography	EXOQuant
4	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Breast cancer cells	MDA-MB-231 - Exo-rich fractions 1-6 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation	EXOQuant
5	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Embryonic kidney cells	HEK293T - Exo-rich fractions 7-10 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography	EXOQuant
6	34108659	Homo sapiens	Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker	Pancreatic cancer cells	PANC-1 - Exo-rich fractions 1-6 pooled	Protein	Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation	EXOQuant